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作物学报 ›› 2021, Vol. 47 ›› Issue (3): 462-471.doi: 10.3724/SP.J.1006.2021.04034

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜耐盐和耐旱相关性状的QTL分析

蒙姜宇(), 梁光伟, 贺亚军*(), 钱伟   

  1. 西南大学农学与生物科技学院, 重庆 400716
  • 收稿日期:2020-02-16 接受日期:2020-10-14 出版日期:2021-03-12 网络出版日期:2020-11-18
  • 通讯作者: 贺亚军
  • 作者简介:E-mail: 1131241810@qq.com
  • 基金资助:
    国家重点研发计划项目(2016YFD0100202);国家自然科学基金项目(31671729);重庆市基础科学与前沿技术研究项目资助(cstc2017jcyjAX0391)

QTL mapping of salt and drought tolerance related traits in Brassica napus L.

MENG Jiang-Yu(), LIANG Guang-Wei, HE Ya-Jun*(), QIAN Wei   

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
  • Received:2020-02-16 Accepted:2020-10-14 Published:2021-03-12 Published online:2020-11-18
  • Contact: HE Ya-Jun
  • Supported by:
    National Key Research and Development Program of China(2016YFD0100202);National Natural Science Foundation of China(31671729);Chongqing Research Program of Basic Research and Frontier Technology(cstc2017jcyjAX0391)

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摘要：

盐胁迫和干旱胁迫是非生物胁迫中影响作物产量的重要因素, 检测与耐盐和耐旱相关的QTL, 可为抗逆油菜品种的选育提供理论依据。本研究利用德国冬性甘蓝型油菜Express和中国半冬性甘蓝型油菜SWU07为亲本构建的包含261个株系的双单倍体(doubled haploid, DH)群体, 分别以1.2% NaCl溶液和20% PEG-6000溶液作为培养液模拟盐胁迫和干旱胁迫, 去离子水为对照, 对2个亲本和DH群体进行发芽试验。播种后7 d测定幼苗根长、鲜重及发芽率, 计算各性状在盐胁迫和干旱胁迫下的相对值, 并作为评价耐盐和耐旱的指标。根据已构建的遗传连锁图谱进行QTL定位。盐胁迫下, 在3次重复中共检测到与盐胁迫相关的QTL 12个, 分布在A02、A03、A05、A09、C01和C09染色体上, 单个QTL可解释的表型变异为3.61%~10.59%, 其中5个QTL在不同的重复中被检测到。干旱胁迫下, 共检测到与干旱胁迫相关的QTL 9个, 分布在A01、A02、A03、A05、A09、A10和C03染色体上, 单个QTL可解释的表型变异为3.94%~12.90%, 其中2个QTL在不同的重复中被检测到。此外, 在A02和A03染色体上检测到与2种胁迫都相关的相互交叠的QTL。这些结果可为油菜耐盐和耐旱性改良提供更多遗传信息。

关键词: 甘蓝型油菜, 耐盐性, 耐旱性, 数量性状位点

Abstract:

Salt and drought stresses are main abiotic stresses affecting crop yield. Detecting the QTLs related to salt and drought tolerance can provide theoretical basis for stress resistance breeding in rapeseed. In this study, a doubled haploid population (DH population), which included 261 lines constructed using the German winter rapeseed cultivar ‘Express’ (female) and the Chinese semi-winter line ‘SWU07’ (male), were used to detect QTLs related to salt and drought tolerance. The seeds were germinated in Petri dishes under 1.2% NaCl and 20% PEG-6000, respectively. The control was under the sterile dH2O. Three tolerance related traits, root length, fresh weight and germination rate of each line were measured on the 7th day after planting. The relative value of root length, fresh weight and germination rate under the salt and drought tolerance condition were calculated. Composite interval mapping (CIM) was used to identify the related QTLs according to the constructed genetic map. A total of 12 QTLs were detected for salt tolerance related traits and located on A02, A03, A05, A09, C01, and C09 chromosome, explaining phenotypic variation ranging from 3.61% to 10.59%. Five of these QTLs were persistently expressed in different repetitions. A total of 9 QTLs were detected for drought tolerance related traits and located on A01, A02, A03, A05, A09, A10, and C03 chromosome, explaining phenotypic variation ranging from 3.94% to 12.90%. Two of these QTLs were persistently expressed in different repetitions. In addition, the overlapped QTLs, located on A02 and A03, were detected under salt stress and drought stress. These results provide more genetic information for improving salt and drought tolerance in rapeseed.

Key words: Brassica napus L., salt tolerance, drought tolerance, QTL

表1

亲本和DH群体在不同胁迫环境下的表型分析"

胁迫环境
Stress environment		 性状
Trait		 亲本 Parent DH群体 DH population
SWU07 表达
Express		 Pt-test 最小值
Min.		 最大值
Max.		 平均值
Mean		 标准差
SD		 变异系数
CV (%)
1.2% NaCl 发芽率 Germination rate 0.036 0.173 0 0 0.590 0.151 0.130 86.333
根长 Root length 0.046 0.032 0.007 0.019 0.313 0.082 0.048 58.970
鲜重 Fresh weight 0.155 0.184 0.028 0.119 0.773 0.272 0.104 38.206
20% PEG-6000 发芽率 Germination rate 0.894 0.959 0.017 0.120 0.980 0.845 0.172 20.377
根长 Root length 0.402 0.694 0 0.242 0.995 0.583 0.157 26.840
鲜重 Fresh weight 0.960 0.744 0.001 0.304 0.993 0.744 0.162 21.824

图1

DH群体耐盐相关性状的频率分布图 a、b、c图分别是盐胁迫下发芽率相对值、根长相对值、鲜重相对值的频率分布图。"

图2

DH群体耐旱性状的频率分布图 a、b、c图分别是旱胁迫下发芽率相对值、根长相对值、鲜重相对值的频率分布图。"

表2

DH群体各性状的相关性分析"


 性状
Trait		 1.2% NaCl 20% PEG-6000
SGR SRL SFW DGR DRL DFW
1.2% NaCl SGR
SRL 0.2564**
SFW 0.1317* 0.4273**
20% PEG-6000 DGR 0.0439 0.1294 0.1817*
DRL 0.0559 0.0166 0.1267 0.3238**
DFW -0.0433 0.0032 0.1729* 0.4494** 0.2656**

表3

DH群体中检测到的耐盐、耐旱相关的QTL"

胁迫环境
Stress
environment		 性状
Trait		 重复
Repetition		 QTL名称
QTL name		 染色体
Chr.		 位置
Position		 加性效应
Additive		 贡献率
R2 (%)		 LOD 值
LOD score		 置信区间
Confidence
interval
1.2% NaCl 发芽率 Germination rate REP1 qSGR-A03-1 A03 27.11 -0.03 7.06 4.13 22.1-32.0
REP1 qSGR-A03-2 A03 35.61 -0.03 4.46 3.07 33.4-38.3
REP1 qSGR-A05 A05 45.11 0.03 3.61 2.53 40.1-52.0
REP1 qSGR-C01-1 C01 37.01 0.04 10.40 4.72 36.0-37.4
REP1 qSGR-C09 C09 8.50 -0.03 3.74 2.61 5.0-16.4
REP 3 qSGR-A03-3 A03 27.11 -0.04 9.37 5.47 22.7-32.0
REP 3 qSGR-A03-4 A03 35.61 -0.03 5.62 3.82 33.4-39.1
REP 3 qSGR-C01-2 C01 37.01 0.04 10.59 4.75 36.0-39.1
根长
Root length		 REP 1 qSRL-A03-1 A03 28.81 -0.01 5.14 3.44 22.3-32.0
REP 1 qSRL-A03-2 A03 35.61 -0.01 3.78 2.50 33.4-39.1
REP 1 qSRL-A09 A09 86.41 -0.01 8.53 5.03 77.8-95.0
REP 1 qSRL-C09-1 C09 7.91 -0.01 6.61 4.31 3.5-11.9
REP 1 qSRL-C09-2 C09 14.91 -0.01 4.99 3.06 11.9-17.5
REP 2 qSRL-C09-3 C09 7.91 -0.03 4.14 2.52 3.0-17.4
REP 3 qSRL-A03-3 A03 30.81 0.26 6.30 3.44 28.4-33.4
鲜重
Fresh weight		 REP 2 qSFW-C09 C09 19.61 0.00 4.64 2.94 17.5-31.8
REP 3 qSFW-A02 A02 46.00 -0.03 5.79 3.52 44.9-50.0
20% PEG-6000 发芽率 Germination rate REP 1 qDGR-A02 A02 47.00 -0.01 4.41 2.57 44.9-51.0
REP 1 qDGR-A03 A03 35.60 0.01 5.45 3.40 33.4-37.0
REP 1 qDGR-A05-1 A05 65.00 0.04 6.05 3.00 59.0-78.6
REP 2 qDGR-A05-2 A05 66.11 0.04 4.90 3.28 59.3-78.1
根长
Root length		 REP 1 qDRL-A02 A02 40.80 0.04 12.90 2.96 40.2-42.1
REP 3 qDRL-A01 A01 10.31 0.16 4.92 2.98 7.9-12.7
REP 3 qDRL-A10 A10 0.51 0.16 4.32 2.62 0-2.7
鲜重
Fresh weight		 REP 1 qDFW-A09 A09 73.41 0.00 7.11 4.43 72.2-74.7
REP 2 qDFW-A01-1 A01 25.50 0.07 3.94 2.51 15.6-37.1
REP 2 qDFW-C03 C03 25.60 -0.06 9.21 3.87 21.4-34.1
REP 3 qDFW-A01-2 A01 25.51 0.07 3.97 2.53 15.6-37.1

图3

耐盐、耐旱相关性状QTL在连锁群上的分布 白色填充的框图代表盐胁迫下检测到的QTL。黑色填充的框图代表干旱胁迫下检测到的QTL。"

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