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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (01): 110-117.doi: 10.3724/SP.J.1006.2013.00110

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of SA Induction on Leaf Cuticular Wax and Resistance to Sclerotinia sclerotiorurn in Brassica napus

NI Yu1,3,WANG Jing1,SONG Chao1,XIA Rui-E1,SUN Zheng-Yuan1,GUO Yan-Jun2,LI Jia-Na1,3,*   

  1. 1 College of Agronomy and Biotechnology, Southwest University; 2 College of Animal Science and Technology, Southwest University; 3 Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China
  • Received:2012-05-01 Revised:2012-10-09 Online:2013-01-12 Published:2012-11-14
  • Contact: 李加纳, E-mail: ljn1950@swu.edu.cn

Abstract:

Cuticular wax on the surfaces of higher plants is believed to be the initial defense system to various pathogens. However, whether or how wax deposition in Brassica napus is involved in the resistant mechanism of infecting Sclerotinia sclerotiorum, is still unclear. In the current experiment, two Brassica napus cultivars with different disease resistances, Zhongshuang 9 (resistant) and Yuyou 19 (susceptible), were selected to analyze the dynamics of disease indices, contents and crystal structure of leaf cuticular wax, activities of defense enzymes, and gas exchange indices under the conditions of SA induction and Sclerotinia sclerotiorurn inoculation. The results showed that SA induction significantly reduced the disease index of Zhongshuang 9, increased its resistance, while SA had no significant influence on disease index of Yuyou 19. When the plants were inoculated with S. sclerotiorurn, the activity of PAL in leaf of Zhongshuang 9 increased while that of Yuyou 19 decreased. The activities of PAL and POD in plants with SA induction were significantly higher than those in plants treated with only S. sclerotiorurn inoculation for both cultivars. The content of total wax in leaf of Zhongshuang 9 was significantly lower than that of Yuyou 19. SA induction increased the contents of total wax and wax constituents for Zhongshuang 9, reduced the amounts of rod crystalloid and increased the amounts of plate crystalloid, and increased the leaf area covered with waxes. The contents of waxes for Yuyou 19 were changed insignificantly by SA induction. However, the changes of the wax crystalloid structure of Yuyou 19 by SA induction were similar with those of Zhongshuang 9, except for a lower leaf area covered with waxes. Conclusion is that both defense enzymes and leaf cuticular waxes are involved in the process of increasing resistance induced by SA for resistant cultivar Zhongshuang 9.

Key words: Brassica napus L, Cuticular wax, Resistance, Salicylic acid, Sclerotinia sclerotiorurn

[1]Jenk M A, Joly R J, Peters P J, Rich P J, Axtell J D, Ashworth E N. Chemically induced cuticle mutation affecting epidermal conductance to water vapor and disease susceptibility in Sorghum bicolor (L.) Moench. Plant Physiol, 1994, 105: 1239–1245



[2]Ficke A, Gadoury D M, Godfrey D, Dry I B. Host barriers and responses to Uncinula necator in developing grape berries. Phytopathol, 2004, 94: 438–445



[3]Russin J S, Guo B Z, Tubajika K M, Brown L, Cleveland T E, Widstorm N W. Comparison of kernel wax from corn genotypes resistant or susceptible to Aspergillus flavus. Biochem Cell Biol, 1997, 87: 529–533



[4]Zinsou V, Wydra K, Ahohuendo B, Schreiber L. Leaf waxes of cassava (Manihot esculenta Crantz) in relation to ecozone and resistance to Xanthomonas blight. Euphytica, 2006, 149: 189–198



[5]Chen Z-Y(陈志谊), Wang Y-H(王玉环), Yin S-Z(殷尚智). A study on the mechanism of resistance to sheath blight in rice. Sci Agric Sin (中国农业科学), 1992, 25(4): 41–46 (in Chinese with English abstract)



[6]Ashraf M, Zafar Z U. Some physiological characteristics in resistant and susceptible cotton cultivars infected with cotton leaf curl virus. Biol Plant, 1999, 42: 615–620



[7]Li H-Y(李海英), Liu Y-G(刘亚光), Yang Q-K(杨庆凯). Studies on the structural resistance to Cercospora sojina Hara in soybean leaves. Chin J Oil Crop Sci (中国油料作物学报), 2002, 24(2): 74–76 (in Chinese with English abstract)



[8]Kang L-G(康立功), Qi F-K(齐凤坤), Xu X-Y(许向阳), Li J-F(李景富). Relationship between tomato leaf wax and cutin layers with infection by helminthosporium carposaprum. China Veget (中国蔬菜), 2010, (18): 47–50 (in Chinese with English abstract)



[9]Luo K(罗宽), Zhou B-W (周必文). Rape Disease and Its Governance (油菜病害及治理). Beijing: China Business Press, 1994 (in Chinese)



[10]Yang B, Rahman M H, Liang Y, Shah S, Kav N N V. Characterization of defense signaling pathways of Brassica napus and Brassica carinata in response to Sclerotinia sclerotiorum challenge. Plant Mol Biol Rep, 2010, 28: 253–263



[11]Guo X M, Stotz H U. Defense against Sclerotinia sclerotiorum in Arabidopsis is dependent on jasmonic acid, salicylic acid, and ethylene signaling. Mol Plant Microbe Interact, 2007, 20: 1384–1395



[12]Zhang W, Yang X F, Qiu D W, Guo L H, Zeng H M, Mao J J, Gao Q F. Pea T1-induced systemic acquired resistance in tobacco follows salicylic acid-dependent pathway. Mol Biol Rep, 2011, 38: 2549–2556



[13]Kacprzak P, Macioszek V K, Kononowicz A K. Induced systemic resistance (ISR) in the protection of plants against pathogenic fungi. Postepy Biol Komorki, 2011, 38: 129–142



[14]Garbay B, Tautu M T, Costaglioli P. Low level of pathogenesis-related protein 1 mRNA expression in 15-day-old Arabidopsis cer6-2 and cer2 eceriferum mutants. Plant Sci, 2007, 172: 299–305



[15]Cajustea J F, González-Candelasa L, Veyrat A, García-Breijo F J, Reig-Arminana J, Lafuentea M T. Epicuticular wax content and morphology as related to ethylene and storage performance of ‘Navelate’ orange fruit. Postharvest Biol Technol, 2010, 55: 29–35



[16]Zhang X-P(臧宪朋), Xu Y-P(徐幼平), Cai X-Z(蔡新忠). Establishment of an inoculation technique system for Sclerotinia sclerotiorum based on mycelial suspensions. J Zhejiang Univ (浙江大学学报), 2010, 36(4): 381–386 (in Chinese with English abstract)



[17]Rosler J, Krekel F, Amrhein N, Schmid J. Maize phenylalanine ammonia-lyase has tyrosine ammonia-lyase activity. Plant Physiol, 1997, 113: 175–179



[18]Olmos E, Piqueras A, Martinez-Solano J R, Hellin E. The subcellular localization of peroxidase and the implication of oxidative stress in hyperhydrated leave of regenerated carnation plants. Plant Sci, 1997, 130: 97–105



[19]Sun N, Song K. Effect of nonthermal treatment on the molecular properties of mushroom polyphenoloxidase. Food Chem Toxicol, 2003, 68: 1639–1643



[20]Kim K S, Park S H, Jenks M A. Changes in leaf cuticular waxes of sesame (Sesamum indicum L.) plants exposed to water deficit. J Plant Physiol, 2007, 164: 1134–1143



[21]Mauch-Mani B, Slusarenko A J. Production of salicylic acid precursors is a major function of phenylalanine ammonialyase in the resistance of Arabidopsis to Peronospora parasitica. Plant Cell, 1996, 8: 203–212



[22]Sreedhara H S, Nandini B A, Shetty S A, Shetty H S. Peroxidase activities in the pathogenesis of Sclerospora graminicola in pearl millet seedlings. Int J Trop Plant Dis, 1995, 13: 19–32



[23]Dietrich R A, Delaney T P, Uknes S J, Ward E R, Ryals J A, Dangl J L. Arabidopsis mutants simulating disease resistance response. Cell, 1994, 77: 565–577



[24]Bowling S A, Clarke J D, Liu Y, Klessig D F, Dong X. The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance. Plant Cell, 1997, 9: 1573–1584



[25]Rate D N, Cuenca J V, Bowman G R, Guttman D S, Greenberg J T. The gain-of-function Arabidopsis acd6 mutant reveals novel regulation and function of the salicylic acid signaling pathway in controlling cell death, defenses, and cell growth. Plant Cell, 1999, 11: 1695–1708



[26]Cao H, Bowling S A, Gordon A S, Dong X. Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell, 1994, 6: 1583–1592



[27]Shah J, Kachroo P, Klessig D F. The Arabidopsis ssi1 mutation restores pathogenesis-related gene expression in npr1 plants and renders defense in gene expression salicylic acid dependent. Plant Cell, 1999, 11: 191–206



[28]Rubiales D, Niks R E. Avoidance of rust infection by some genotypes of Hordeum chilensedue to their relative inability to induce the formation of appressoria. Physiol Mol Plant Pathol, 1996, 49: 89–101



[29]Tsuba M, Katagiri G, Takeuchi Y, Yamaoka N. Chemical factors of the leaf surface involved in the morphogenesis of Blumeria graminis. Physiol Mol Plant Pathol, 2002, 60: 51–57



[30]Furtado G Q, Alves S A M, Godoy C V, Salatino M L F, Massola N S. Influence of light and leaf epicuticular wax layer on phakopsora pachyrhizi infection in soybean. Trop Plant Pathol, 2009, 34: 306–312



[31]Shepherd T, Griffiths D W. The effects of stress on plant cuticular waxes. New Phytol, 2006, 171: 469–499



[32]Li J-J(李婧婧), Huang J-H(黄俊华), Xie S-C(谢树成). Plant wax and its response to environmental conditions: an overview. Acta Ecolo Sin (生态学报), 2011, 31(2): 565–574 (in Chinese with English abstract)



[33]Koch K, Hartmann K D, Schreiber L, Barthlott W, Neinhuis C. Influences of air humidity during the cultivation of plants on wax chemical composition, morphology and leaf surface wettability. Environ Exp Bot, 2006, 56: 1–9



[34]Guo Y-J(郭彦军), Ni Y(倪郁), Guo Y-J(郭芸江), Han L(韩龙), Tang H(唐华). Effects of air humidity and soil water deficit on characteristics of leaf cuticular waxes in alfalfa (Medicago staiva).Acta Ecolo Sin (生态学报), 2011, 31(18): 5273–5280 (in Chinese with English abstract)



[35]Smith J A, Blanchette R A, Burnes T A, Gillman J H, David A J. Epicuticular wax and white pine blister rust resistance in resistant and susceptible selections of eastern white pine (pinus strobus). Phytopathol, 2006, 96: 171–177



[36]Raffaele S, Vailleau F, Leger A, Joubes J, Miersch O, Huard C, Blee E, Mongrand S, Domergue F, Roby D. A MYB transcription factor regulates very-long-chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis. Plant Cell, 2008, 20: 752–767



[37]José J R, Alexander Y. Surface lipids and plant defenses. Plant Physiol Bioch, 2009, 47: 540–549

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