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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1224-1232.doi: 10.3724/SP.J.1006.2016.01224

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Dynamics in Contents of Different Types of Polyamine in Wheat Embryos and Its Relationship with Resistance to Drought Stress

DU Hong-Yang1,2,LIU Gu-Ting3 YANG Qing-Hua2,LIU Huai-Pan1,2,*   

  1. 1College of Life Science and Agronomy / Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou Henan 466001, China; 2 The College of Agronomy / The Collaborative Innovation Center of Henan Grain Crops, Zhengzhou 450002, Henan, China; 3College of Biological Science, China Agricultural University , Beijing 100193, China
  • Received:2015-12-03 Revised:2016-05-09 Online:2016-08-12 Published:2016-05-23
  • Contact: 刘怀攀, Email: liuhuaipan2013@126.com, Tel: 0394-8178138 E-mail:duhongyang2005@126.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31271627), the Start-up Fund for High-level Scholars of Zhoukou Normal University (ZKNU2015109), and the China Special Fund for Agro-Scientific Research in the Public Interest (201203077).

Abstract:

This study aimed at understanding the functions of different kinds of polyamine in developing embryos of wheat under drought stress. The drought resistant variety “Luomai 22” and the drought-sensitive variety “Yumai 48” were treated for 10 days with drought stress (DS) and DS plus exogenous polyamines (Spd and Spm) and inhibitors (MGBG and O-Phen) on the 10th day after anthesis. In both varieties, the levels of free Put, Spd and Spm and the content of acid soluble covalently conjugated polyamines (ASCC-PA) increased under DS treatment, particularly, the free Put content in Yumai 48 increased rapidly and continuously during 10 days after treatment. The increased magnitude of free Spd and Spm contents in Luomai 22 embryos was similar to that in Yumai 48 embryos at the early stage of DS, but significantly larger than that in Yumai 48 embryos at later stage of DS. The content of acid insoluble covalently conjugated Put (AISCC-Put) was low in both varieties at early stage of DS, however, increased obviously in Luomai 22 at later stage of DS, with a significantly higher level in Luomai 22 than in Yumai 48. Application of exogenous Spd or Spm resulted in significantly increased free Spd and Spm contents in Yumai 48 at later DS stage; simultaneously, the relative water content of flag leaf and the relative dry matter increase rate of grain increased and the relative plasma membrane permeability of flag leaf decreased, indicating improved drought resistance in Yumai 48. Polyamine inhibitors showed negative effects on drought resistance, in which MGBG had a strong inhibition on the conversion from free Put to free Spd or Spm in embryos of Luomai 22 and O-Phen depressed AISCC-Put induction by drought stress. These results suggest that drought tolerance of wheat might be improved by the easy conversion from free Put to free Spd, Spm, or AISCC-Put after flowering.

Key words: Drought stress, Wheat grain embryo, Polyamines

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