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    Effects of sowing date on the yield of different late rice variety types and its relationship with the allocation of temperature and light resources during the whole growth period of rice in the lower reaches of the Yangtze River
    FENG Xiang-Qian, YIN Min, WANG Meng-Jia, MA Heng-Yu, LIU Yuan-Hui, CHU Guang, XU Chun-Mei, ZHANG Xiu-Fu, WANG Dan-Ying, ZHANG Yun-Bo, CHEN Song
    Acta Agronomica Sinica    2022, 48 (10): 2597-2613.   DOI: 10.3724/SP.J.1006.2022.12072
    Abstract87)   HTML12)    PDF(pc) (2930KB)(135)       Save

    The yield, physiological characteristics, temperature, and light configurations of different late season rice varieties under different sowing treatments were studied to explore the suitable variety types of late japonica rice and their suitable sowing periods in the lower reaches of the Yangtze River. Two indica rice (CK), seven inbred japonica rice and three indica-japonica hybrid rice were used, and the sowing date adjustment experiment was carried out in the local suitable sowing period. The sowing treatments were set evenly within the locally available sowing period (mid-June to early July), with sowing periods set at 0 days (I), +T days (II), +2T days (III), +3T days (IV), and +4T days (V), respectively. The result showed that the total growing period of indica rice was slightly extended with delayed sowing, mainly due to the extension of the grain filling period. The grain filling period of japonica rice was also extended, but the total growing period was slightly shortened due to the greater shortening of the vegetative growth period. The yield of hybrid indica-japonica rice and inbred japonica rice fluctuated slightly with delayed sowing. The yield of inbred japonica rice [8.0±0.5 (t hm-2)] was basically stable under five sowing dates. The yield of hybrid indica-japonica rice increased with delayed sowing dates, and peaked at sowing stage IV and sowing stage III in 2019 and 2020, respectively, with the highest yields of 11.0 t hm-2 and 9.1 t hm-2. The yield of indica rice varieties decreased significantly with the delay of sowing period. At the same time, yield differences among different variety types gradually increased with sowing delay, with yield differences increasing from 0.5 t hm-2 to 5.1 t hm-2. The effects of sowing changes on yield components varied. The number of effective panicles increased with sowing delay, the number of grains per panicle, and the grain filling rate decreased to different degrees, while the 1000-grain weight did not change significantly. The principal component analysis at different sowing dates revealed that with sowing delay the differences in variety characteristics evolved from differences in sink-source synergism to differences in sink-source synergism superimposed on temperature and light resource allocation, and then to differences in low temperature tolerance at later stages. As a result, indica rice should be sown appropriately early to make full use of the early temperature and light resources to achieve high yield by producing adequate panicles. Japonica rice possessed strong sowing adaptability, and indica-japonica hybrid rice had advantages in yield under all sowing periods. In the case of late harvest of the previous crop, the selection of indica-japonica hybrid rice varieties was more yield guarantee, while the selection of the best sowing period could maximize the yield of indica-japonica hybrids.

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    Insights on developing modern corn ecological breeding in southwest China
    PAN Guang-Tang, YANG Ke-Cheng, GAO Shi-Bin
    Acta Agronomica Sinica    2022, 48 (10): 2427-2434.   DOI: 10.3724/SP.J.1006.2022.13065
    Abstract137)   HTML18)    PDF(pc) (295KB)(128)       Save

    Based on the principle of interaction between genotype and environment (G × E), we analyze the ecological zoning characteristics of maize breeding in southwest China, the development demand of national economy, the development trend of maize industry, the change of breeding target demand, the innovation of breeding technology system and so on. We focus on the systematic summary and explanation of the connotation of maize ecological breeding in southwest China in the new period. Further condensed with “full attention and utilization of (G × E) interaction” as the core, “industrialization of breeding objectives, diversification of variety types, special identification of variety selection, regionalization of variety layout, and collectivization of variety promotion.” the development concept of “modern ecological breeding” in Southwest corn region with the main content of “model of variety cultivation” systematically put forward the breeding goal of “low risk and high efficiency new hybrid” in the new period, which is characterized by “stable yield, high efficiency, environment-friendly, high quality and safety, easy seed production and harvest by machine ”. In order to promote the sustainable and healthy development of maize production and industry in southwest China, we proposed the following aspects should be emphasized in breeding technology system: 1) the path is to improve disease resistance and stress resistance of new varieties based on the premise of fine exploration and accurate identification of excellent and specific breeding resources; 2) the key is to utilize different heterotic groups, especially the cultivation of excellent inbred lines of tropical germplasm groups urgently needed in different ecological regions, and the identification of new hybrid combinations in specific ecological regions under multi-point biotic and abiotic strong stress for multi-year; 3) the guarantee is to popularize new varieties with stable yield, high yield and high efficiency guaranteed by efficient utilization of resources in specific ecological regions, environment-friendly and sustainable development of maize production.

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    Milk line changes of maize grain and the relationship with grain filling and drying process
    LI Hong-Yan, ZHOU Lin-Li, GAO Shang, XUE Jun, MING Bo, ZHAO Ru-Lang, WANG Ke-Ru, XIE Rui-Zhi, HOU Peng, WANG Yong-Hong, LI Shao-Kun
    Acta Agronomica Sinica    2022, 48 (10): 2560-2566.   DOI: 10.3724/SP.J.1006.2022.13054
    Abstract70)   HTML7)    PDF(pc) (1189KB)(120)       Save

    In order to clarify the relationship between milk line proportion and grain moisture content and grain filling process and provide references for the decision-making of maize harvest period, a field observational experiment was conducted with 9 spring-sown maize varieties in 2017 and 2018 at Yongning County, Yinchuan City, Ningxia Hui Autonomous Region. In this study, the cross-sectional images, grain moisture content, and 100-grain dry weight were measured continuously on the central part of the ear within 20 to 80 days after silking. An image-assisted software tool was designed to measure the milk line proportion of maize grains, accurately obtaining the information of milk line proportion in different varieties or different growth stages. Regression analysis showed that there were slight differences in the variation of milk line proportion, grain moisture content, and filling process between varieties and inter-years, which generally conformed to the same variation regulations. There was a significant linear relationship between milk line proportion and grain moisture content, and the regression equation was y= -0.2572x+ 52.482. The relationship between milk line proportion and grain filling process was fitted to Richards curve significantly, and the regression equation was y = 99.65/[1+exp(2.45-0.07x)](1?3.70). The changes of milk line proportion were closely related to grain moisture content and grain filling process within a certain range, which can be used as one of the field evaluation indicators for different kinds of maize harvest.

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    Regulation of adventitious root development by HvLBD19 gene in barley ( Hordeum vulgare L.)
    GUO Bao-Jian, WANG Shuang, LYU Chao, WANG Fei-Fei, XU Ru-Gen
    Acta Agronomica Sinica    2022, 48 (10): 2435-2442.   DOI: 10.3724/SP.J.1006.2022.11088
    Abstract131)   HTML20)    PDF(pc) (5864KB)(109)       Save

    LBD gene family is a plant specific transcription factor family, which plays an important role in regulating plant development and nitrogen metabolism. Micro-synteny analysis showed that barley HvLBD19 was an orthologous of maize RTCS and rice ARL1 gene. Temporal and spatial expression analysis revealed that this gene was most abundant in adventitious roots and was induced by exogenous auxin treatment, which encoded a protein located in the nucleus. Transgenic results indicated that the adventitious root length increased by nearly one time, and the number of adventitious roots increased by 40%. It was preliminarily confirmed that HvLBD19 gene controlled adventitious root development in barley and provided a foundation for further research on the molecular mechanism of HvLBD19 gene in regulating adventitious root development in barley.

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    A critical review on the principles and procedures for cultivar development and evaluation
    YAN Weikai
    Acta Agronomica Sinica    2022, 48 (9): 2137-2154.   DOI: 10.3724/SP.J.1006.2022.11105
    Abstract163)   HTML11)    PDF(pc) (890KB)(328)       Save

    Plant breeding plays an indispensable role in meeting the increasing need for food and comfort of the mankind in a changing climate. Numerous concepts and procedures aiming at improving breeding efficiency have been put forward, such as various strategies for dealing with genotype by environment interaction, stability analyses, mega-environment analyses, experimental designs and analyses, biplot analyses, and selection indices. In addition, genomic selection has evolved into a stage that plant breeders must consider and cannot ignore. It is of practical importance to understand the relationships among these concepts and procedures, their usefulness, drawbacks, and pitfalls, as well as their place in the theoretical framework of plant breeding and genotype evaluation. Based primarily on personal research and experience, this article attempted to develop a systematic narrative on the principles, key concepts, and analytic procedures related to cultivar development and evaluation, with demonstrations using real-world data.

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    Characterization and Functional Analysis of a Small GTP-binding Protein AtRAB Interacting with H +-Pyrophosphatase AVP1 in Arabidopsis thaliana
    LIU Rong-Bang,CHEN Ming,GUO Meng-Meng,SI Qing-Lin,GAO Shi-Qing,XU Zhao-Shi,LI Lian-Cheng,MA You-Zhi,YIN Jun
    Acta Agron Sin    2014, 40 (10): 1756-1766.  
    Abstract627)   HTML0)    PDF(pc) (6116KB)(897)       Save

    H+-Pyrophosphatase (H+-PPase) is an important proton transporter in plants. It cooperates with H+-ATPase and transport protons in vacuole or extracellular area to maintain a constant H+ gradient, which enables the transport of ions and other components (e.g. amino acids, carbohydrates). In the current research, AVP1 was applied to membrane proteins-based yeast two-hybrid system, and a small GTP-binding protein AtRAB was identified by screening the Arabidopsis cDNA library. The interaction between AVP1 and AtRAB was confirmed by interaction analysis in yeast. Bimolecular fluorescent complementation (BiFC) analysis suggested that AtRAB and AVP1 interaction took place in the plasma membrane and the nucleus. Phenotypes of wild type (WT) and Arabidopsis mutants of avp1 (AVP1) and rab (AtRAB) were compared under high-salt, low-phosphorus and low-potassium conditions. Under the high salt stress, avp1 and rab presented similar phenotypes along with the increase of NaCl concentration, which were characterized by shortened root length and reduced lateral root number in comparison with the WT. The decreasing phosphorus concentration also led to the phenotypes of shortened root length, reduced lateral root number and reduced total root area in the two mutants. Particularly, stronger response was observed in rab than in avp1 under the same phosphorus concentration. The potassium treatments resulted in similar phenotypes to those in phosphorus treatment. The results indicated that AVP1 can interact with AtRAB on the plasma membrane and the nucleus and further influence the absorption of ions in plants. The two mutants of avp1 and rab showed similar phenotypes, suggesting that both AVP1 and AtRAB positively regulate plant response to high salt, low phosphorus and low potassium stresses in the same signaling pathway.

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    Response of grain yield formation to planting density of maize varieties in different eras
    WANG Li-Qing, YU Xiao-Fang, GAO Ju-Lin, MA Da-Ling, HU Shu-Ping, GUO Huai-Huai, LIU Ai-Ye
    Acta Agronomica Sinica    2022, 48 (10): 2625-2637.   DOI: 10.3724/SP.J.1006.2022.13057
    Abstract73)   HTML7)    PDF(pc) (884KB)(97)       Save

    To clarify the difference of photosynthetic and grain-filling characteristics of maize varieties in different ages in response to increasing planting density, and to provide a theoretical basis for the reasonable density and production of maize, in this study, five maize varieties planted in large areas during production from 1970s to 2010s in China were used as materials, two planting densities of 45,000 plants hm-2 and 105,000 plants hm-2 were set for three-year field experiment. The yield and its components, leaf photosynthetic performance, and grain filling characteristics of maize varieties in different years were compared and analyzed under different planting densities. The results showed that under low planting density, compared with varieties of 1970s-2000s, the SPAD value of ear-leaf after anthesis, Pn of ear-leaf at 50 d after anthesis, and the average grain filling rate of 2010s varieties (DH618) were significantly increased by 2.18-12.05, 0.57-4.88 μmol CO2 m-2 s-1, and 0.02-0.09 g kernel-1 d-1. The active grain-filling period was significantly prolonged by 2.62-4.74 d, which significantly increasing grain weight and yield at P<0.05. After densification, the yield of varieties in 1970s and 1980s decreased, the yield of varieties in the 1990s did not change significantly, but the yield of varieties in 2000s and 2010s increased significantly and the largest increased in 2010s was 2.11 t hm-2. Further correlation analysis to the indicators of photosynthetic performance, filling characteristics, 100-grain weight, and yield, the results showed that 100-grain weight of 2010s varieties under high planting density was more dependent during active grain filling period (r = 0.70). Compared with 1970s to 2000s, the 2010s varieties kept relatively high SPAD at silking stage, milking stage, and maturity stage (increased by 6.42%-41.92%), delayed leaf senescence and maintained high LAD in maize leaves after anthesis and Pn in 30-50 d after anthesis (increased by 1.09%-88.95%). Therefore, more photosynthates were accumulated, which prolonged the active grain filling period by 8.73% to 15.80% and the average filling rate increased by 3.39% to 24.33%, and then maintained a relatively stable 100-grain weight.

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    Role of complementarity and select effect for yield advantage of maize/legumes intercropping systems
    ZHAO Jian-Hua, SUN Jian-Hao, CHEN Liang-Zhi, LI Wei-Qi
    Acta Agronomica Sinica    2022, 48 (10): 2588-2596.   DOI: 10.3724/SP.J.1006.2022.13063
    Abstract87)   HTML4)    PDF(pc) (518KB)(97)       Save

    Competition and complementarity is one of main ecological principle for productivity advantage of intercropping system. To determine the effect of time niche differentiation on yield of intercropped species and the advantage of intercropping systems, a four-year field experiment was conducted during 2017-2020 in Zhangye city, Gansu province, China. The object of this study is to determine the role of complementarity effect and selection effect for yield advantage of maize/legumes intercropping systems. The experiment included 3 maize/legume intercropping systems of maize/pea (M/P), maize/faba bean (M/F), and maize/soybean (M/S), and 4 sole crops systems of sole pea (SP), sole faba bean (SF), sole soybean (SS), and sole maize (SM). The yield intercropped crops in different plant patterns were investigated, the overyielding of intercropped crops, aggressivity of legume relative to maize, land equivalent ratio (LER), net effect (NE), complementarity effect (CE), and selection effect (SE) were analyzed. The results showed that on average four years, the LER value of M/P, M/F, and M/S were 1.30, 1.31, and 1.13, respectively. The partial land equivalent ratio value of soybean was blow 0.5, the partial land equivalent ratio value of pea and faba bean were above 0.5, and also the partial land equivalent ratio value of maize was above 0.5. The overyielding of pea, faba bean, and soybean were 53.3%, 81.4%, and -14.9%, respectively. The overyielding of maize intercropped with pea, faba bean, and soybean were 13.0%, -5.8%, and 32.3%, respectively. The aggressivity of pea and faba bean relative to maize were above 0, the aggressivity of soybean relative to maize were below 0. The complementarity effect of M/P and M/F were significantly higher than that of M/S, whereas, the selection effect of M/S was greater than those of M/P and M/F. A positive correlation was observed between LER and CE, and a negative correlation was observed between LER and SE. A positive correlation was observed between the overyielding of legumes and SE. In conclusion, yield advantage of M/P and M/F was mainly result from the CE, yield advantage of M/S was mainly result from the SE.

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    Effect of Ridge & Terraced Cultivation on Rice Yield and Root Trait
    ZHENG Hua-Bin,YAO Lin,LIU Jian-Xia,HE Hui,CHEN Yang,HUANG Huang
    Acta Agron Sin    2014, 40 (04): 667-677.   DOI: 10.3724/SP.J.1006.2014.00667
    Abstract885)   HTML1)    PDF(pc) (15075KB)(4512)       Save

    A field experiment was conducted in Changsha city of Hunan Province in 2011 and 2012 to find out a cultivation technique of ridge & terraced cultivation (RTC) for improving rice yield and root trait. Super hybrid rice Yliangyou 1, hybrid rice Shanyou 63 and conventional rice Huanghuazhan were used in the mid-rice season. The cultivation patterns were designed as ridge & terranced cultivation (T1, ridge width 60 cm; T2, ridge width 120 cm), bed cultivation (T3, bed width 70 cm; T4, bed width 140 cm; T5, bed width 230 cm), and traditional cultivation (T0). The characteristics of grain yield formation and root character under different cultivation patterns were analyzed. Results showed that ridge & terraced cultivation (T1) and bed cultivation (T3) could increase grain yield compared with traditional cultivation (T0), T1 had the highest yield, which was 22.2% (17.1%–27.2%) higher than that of T0, the next was T3, with the yield 10.4% (5.8%–15.0%) higher than that of T0. However, increasing range of yield declined gradually with the increase of ridge width or bed width. The important reason for increasing yield by using the two cultivation techniques was higher effective panicles and spikelets per panicles, better root trait (root oxidation ability, root surface absorption area and soil porosity) and higher dry matter accumulation after full heading stage. Meanwhile, root anatomical structureshowed that the thickness of root cortex was decreased and the size of root vascular cylinder and vessel areas was increased in RTC, so that the capacity of water absorption was enhanced.

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    Cited: Baidu(2)
    Dissecting the genetic basis of kernel size related traits and their combining ability based on a hybrid population in maize
    LI Ting, WANG Ya-Peng, DONG Yuan, GUO Rui-Shi, LI Dong-Mei, TANG Ya-Ling, ZHANG Xing-Hua, XUE Ji-Quan, XU Shu-Tu
    Acta Agronomica Sinica    2022, 48 (10): 2451-2462.   DOI: 10.3724/SP.J.1006.2022.13052
    Abstract96)   HTML14)    PDF(pc) (665KB)(84)       Save

    Combining ability is a crucial index for evaluating elite inbred lines and selecting superior hybrids during maize breeding processes. Kernel size related traits are important components of grain yield. Therefore, dissecting the genetic basis of kernel size related traits and their combining ability is beneficial for improving maize yield. In this study, we performed genome-wide association study (GWAS) of kernel size related traits and their combining ability using 246 hybrids breed by designed as NCII mating. The broad-sense heritability of kernel length (KL), kernel width (KW), and kernel thickness (KT) was 76.20%, 86.52%, and 81.14%, respectively. The correlation coefficient between each trait and their combining ability was more than 0.58, indicating there was significant positive correlation. Based on the efficient mixed-model association expedited (EMMAX) algorithm, we identified 31, 21, and 5 significant single nucleotide polymorphisms (SNPs) associated with the best linear unbiased estimation (BLUE), general combining ability (GCA), and special combining ability (SCA) of kernel size related traits, respectively. Among these significant SNPs, 10 SNPs were co-located both kernel size related traits and their combining ability. The effect analysis of co-located significant SNPs showed that three SNPs were additive, four were partial dominance and one was overdominance. Further, 17 candidate genes were predicted from candidate regions of co-located SNPs or SNPs with large effect, such as shrunken 1, emp6 and so on. The results further dissect the genetic architecture of maize kernel size related traits and their combining ability and provide useful information for improving grain yield in maize breeding.

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    Effects of different amount of drip irrigation on carbon metabolism and photosynthetic nitrogen utilization efficiency of maize after anthesis under shallow buried drip irrigation
    YANG Heng-Shan, ZHANG Yu-Shan, GE Xuan-Liang, LI Wei-Min, GUO Zi-He, GUO Nuan
    Acta Agronomica Sinica    2022, 48 (10): 2614-2624.   DOI: 10.3724/SP.J.1006.2022.13061
    Abstract52)   HTML4)    PDF(pc) (723KB)(78)       Save

    The objective of this study is to explore the effects of different amount of drip irrigation on carbon metabolism and photosynthetic nitrogen use efficiency of maize after anthesis under shallow buried drip irrigation. Three treatments of irrigation amount 40% (W1: 1600 m3 hm-2), 50% (W2: 2000 m3 hm-2), and 60% (W3: 2400 m3 hm-2) of traditional border irrigation (4000 m3 hm-2) were set to study dynamic characteristics of leaf source, photosynthesis, activities of photosynthetic carbon metabolism enzyme, photosynthetic nitrogen use efficiency, content of non-structural carbohydrate after anthesis, and yield of different amount drip irrigation under shallow buried drip irrigation. The results showed that there was no significant difference on mean yield between CK and W3, while W2 and W1 were both significantly lower than CK and the annual average yield of W2 and W1 respectively decreased by 3.91% and 11.18% compared with that of CK from 2018 to 2020. There was also no significant difference on yield W3 and W2, while W3 was significantly higher than W1. Compared with W1, the annual yield of W3 increased by 17.56%, 9.06%, and 9.56%, respectively. The instantaneous photosynthetic efficiency, instantaneous water use efficiency, and instantaneous carboxylation rate of W3 and CK were both higher at flowering stage and was the lowest in W1, while the performance of stomatal limit was the opposite. The leaf source characteristics and activities of photosynthetic carbon metabolism enzyme of W2 and W1 were both lower than W3 from 30 days after anthesis to maturity, in which the leaf area, specific leaf quality, content of chlorophyll, and LAD of W3 increased by 20.29%, 3.03%, 14.80%, and 21.37%, respectively. Compared with CK, the activities of 1,5-diphosphate ribulose carboxylase, pyruvate phosphate double kinase, malase, malate dehydrogenase, and phosphoenolpyruvate carboxylase of W3 increased by 19.66%, 12.53%, 10.67%, 21.17%, and 11.72%, respectively. There was no significant difference between the photosynthetic nitrogen use efficiency of CK and W3, which were both higher than W1 from 20 to 50 days after anthesis. Compared with W2, W1, and CK from 50th day after anthesis to maturity, the photosynthetic nitrogen use efficiency of W3 increased by 3.24%, 3.29%, and 7.40%, respectively. Compared with W2, CK, and W1, the sucrose content of W3 were the highest from the 50th day after anthesis to maturity of which increased by 11.31%, 14.02%, and 43.48% respectively. Compared with W2, CK and W1 from the 30th day after anthesis to maturity, the soluble sugar content of W3 was the highest from the 30th day after anthesis to maturity and W3 increased by 14.06%, 17.78%, and 34.20%, respectively. Compared with CK, W2, and W1, the starch content of CK had no significant difference with W3, which was the highest from the 50th day after anthesis to maturity, increased by 3.35%, 7.58%, and 24.93%, respectively. Consequently, when irrigation amount reached to 60% of normal irrigation amount of traditional border irrigation, maize under the shallow buried drip irrigation had better leaf source characteristics, strong activities of photosynthetic carbon metabolism enzyme, photosynthetic nitrogen use efficiency, non-structural carbohydrate content and yield, which could provide theoretical guide for rational irrigation, water saving and yield stability of maize.

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    Difference of Amylopectin Structure among Various Rice Genotypes Differing in Grain Qualities and Its Relation to Starch Physicochemical Properties
    HE Xiao-Peng,ZHU Chang-Lan,LIU Ling-Long,WANG Fang,FU Jun-Ru,JIANG Ling
    Acta Agron Sin    2010, 36 (2): 276-284.   DOI: 10.3724/SP.J.1006.2010.00276
    Abstract2431)      PDF(pc) (355KB)(2806)       Save

    As the main constituents of rice starch, rice amylopectin and its structure is one of major determinants for the development of starch granule structures and starch physicochemical properties. Understanding amylopectin chain length distribution properties in different rice varieties, and clarifying the correlations between the amylopectin structure and rice starch physicochemical properties, may provide theoretical basis for revealing the mechanism of rice quality development and guide rice quality improvement. In this study, the amylopectin structure of some rice varieties in China and some introduced rice varieties with different qualities were characterized by improved fluorophore-assisted carbohydrate electrophoresis (FACE) method based on DNA sequencer. On the basis of eliminating the impact of amylose content (AC), the correlations between amylopectin structure and rice starch physical chemistry properties were analyzed. The results indicated that rice amylopectin structure in 50 varieties could be classified into I-type and II-type, according to the amylopectin chain ratio of åDP£11/åDP£24. The amylopectin chain ratio of the I-type amylopectin is less than 0.22, whereas the II-type is higher than 0.26. All the 15 japonica varieties’ amylopectin belonged to II-type. Among the 35 indica varieties, for amylopectin structure, 15 belonged to I-type and 20 belonged to II-type. The SDP6-11 and amylopectin chain ratio had very significantly negative correlations (P<0.01) with pasting temperature (PT) and relative crystallinity (RC). The åDP28-34 had very significantly negative correlations with PT, but did not have significantly correlations with RC. The åDP13-24 and åDP39-49 had very significantly positive correlations with PT and significantly positive correlations (P<0.05) with RC. In glutinous rice, low AC and high AC varieties, the correlations of SDP6-11, åDP28-34, amylopectin chain ratio with PT and RC were essentially consistent with these in all varieties. The correlations between åDP28-34 and PT in low AC, between åDP39-49 and PT in glutinous rice and high AC varieties, between åDP39-49 and RC in high AC varieties were consistent with these in all varieties. The proportion of chain amounts in amylopectin with different chain length ranges was not significantly correlated with GC and RVA profile properties. Therefore, the proportion of chain amounts in amylopectin with different chain length ranges has relation to rice starch PT and RC, but not close relation to GC and RVA profile characteristics in most cases. The short chain amounts of DP6-11 have a function of decreasing rice starch PT and RC, the medium chain amounts of DP13-24 have a function of increasing rice starch PT and RC, the relatively long chain amounts of DP 28-34 have a function of decreasing rice starch PT and the long chain amounts of DP 39-49 have a function of increasing rice starch PT and RC.

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    Cited: Baidu(19)
    Development and Application of the Functional Marker for Imidazolinone Herbicides Resistant ALS Gene in Rice
    Fang-Quan WANG, Jie YANG, Fang-Jun FAN, Wen-Qi LI, Jun WANG, Yang XU, Jin-Yan ZHU, Yun-Yan FEI, Wei-Gong ZHONG
    Acta Agronomica Sinica    2018, 44 (03): 324-331.   DOI: 10.3724/SP.J.1006.2018.00324
    Abstract648)   HTML12)    PDF(pc) (7415KB)(826)       Save

    Breeding and utilization of herbicide resistant rice are significant to rice production. By screening the rice germplasm, we found the herbicide resistant material “Jinjing 818”. An SNP mutation G to A was present in Acetolactate synthase (ALS) gene at 1880 bp position, leading to the alteration from serine (S, AGT) to asparagine (N, AAT), which confers herbicide resistance. In this study, 11 allelic-specific PCR (AS-PCR) primers were designed based on the functional mutation. After optimized these primers, we obtained two primer combinations F1N (S1/S9) and F1M (S1/S10), named AS-ALS marker. Using this marker detected the genetic population, its parents, F1 hybrid, F2 and also rice collections, inbred lines, showing that the herbicide susceptibleness allelic ALS-G could be amplified by F1N, the herbicide resistance allelic ALS-A by F1M, and heterozygous genotype by F1N and F1M simultaneously. The genotype of those tested materials perfectly matched with the phenotype of herbicide resistance or susceptibleness. Aided by AS-ALS marker selection, the homozygous ALS-A pedigrees in multi-generation backcross or self-cross showed stable herbicide resistance. Therefore, the allelic-specific PCR functional marker AS-ALS can be used in herbicide breeding efficiently, also screening herbicide resistant rice germplasm. In conclusion, the AS-ALS marker developed in this research is inexpensive and effective in breeding practice.

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    Genome-wide identification and expression analysis of potato PYL gene family
    JIA Xiao-Xia, QI En-Fang, MA Sheng, HUANG Wei, ZHENG Yong-Wei, BAI Yong-Jie, WEN Guo-Hong
    Acta Agronomica Sinica    2022, 48 (10): 2533-2545.   DOI: 10.3724/SP.J.1006.2022.14183
    Abstract68)   HTML7)    PDF(pc) (1785KB)(67)       Save

    As a key signalling molecule, abscisic acid (ABA) extensively regulates plant growth and development, and stress response processes through its core signalling pathway PYLs-PP2Cs-SnRK2s. As a core component of ABA signalling transduction, PYLs protein plays an irreplaceable role. In order to explore the evolution and expression pattern of PYL (PYR/PYL/RCARs) genes in potato, 17 PYL genes were identified from the whole potato genome ‘DM-v 6.1’, and their distribution, protein physical and chemical properties, system evolution, structural characteristics, and the relative expression patterns were analysed. The results showed that the 17 StPYL genes were unevenly distributed on 8 chromosomes, with amino acids of 163-231 aa, isoelectric points of 4.5-8.6, and relative molecular weights of 18.71-25.29 kD. According to the gene structure and protein phylogenetic characteristics, the members of the StPYL family were divided into three subgroups. Motif 1 existed in all genes in this family, indicating that it was relatively conserved in the evolution of StPYLs. Gene expression patterns showed that the members of the StPYL family had obvious tissue expression specificity, and except for the up-regulation of StPYL1 under exogenous hormones (BAP, ABA, and IAA) and abiotic stress (high temperature, salt and drought), the rest of the StPYL genes were functionally differentiated and their relative expression patterns were different under different stresses. The results of this study lay a theoretical foundation for further elucidating the function of StPYLs gene in potato.

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    Genome-wide identification of the Class III PRX gene family in foxtail millet ( Setaria italica L.) and expression analysis under drought stress
    MA Xin-Lei, XU Rui-Qi, SUO Xiao-Man, LI Jing-Shi, GU Peng-Peng, YAO Rui, LIN Xiao-Hu, GAO Hui
    Acta Agronomica Sinica    2022, 48 (10): 2517-2532.   DOI: 10.3724/SP.J.1006.2022.14185
    Abstract77)   HTML9)    PDF(pc) (2102KB)(66)       Save

    Class III peroxidases (PRX) are a family of plant-specific peroxidases that play an important role in plant growth and development as well as in abiotic stresses. Foxtail millet (Setaria italica L.), as a C4 plant, is a model plant for stress resistance. However, the function of class III peroxidases family genes is rarely reported in foxtail millet. A genome-wide expression analysis was conducted to investigate the expression pattern of class III peroxidase gene family (SitPRXs) under drought stress and ABA induction. In this study, 132 members of the Class III PRX gene family were identified in the whole genome in foxtail millet by bioinformatics and named SitPRX1-SitPRX132 according to their chromosomal position. 132 members were classified into Mα, Mβ, Mγ, MIKCC, and MIKC* subfamilies by phylogenetic analysis of foxtail millet, Arabidopsis, and rice. Gene structure and conserved motif analysis indicated a high level of conservation in the same subfamily. Gene duplication analysis revealed fragmental duplication in 17 SitPRX genes (13%) and tandem duplication in 78 SitPRX genes (59%), thus tandem duplication events playing an important role in SitPRX gene amplification. Interspecies homology analysis with Arabidopsis, rice and maize revealed that most SitPRXs were formed after dicotyledonous and monocotyledonous plants diverged. Transcriptome analysis implied that members of the SitPRX gene family were differentially expressed in seedlings, roots, stems, and leaves, as well as in panicles in foxtail millet. Analysis of promoter cis-acting elements showed that 79 SitPRXs contained cis-acting elements associated with drought stress response, and further qRT-PCR analysis showed that SitPRX12, SitPRX41, SitPRX81, SitPRX110, and SitPRX126 were induced to be expressed by PEG and ABA, suggesting that these genes may be regulated through an ABA-dependent signalling pathway in response to drought stress and could be used as these genes may be candidates for further studies on the drought resistance function of the class III PRX gene family. These results of this study provide new information for the comprehensive analysis of the structure and biological functions of SitPRX genes, the molecular mechanism of drought resistance, and molecular breeding in foxtail millet, with a view of providing ideas for the breeding of new varieties of highly efficient stress-resistant crops in the future.

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    Effects of planting methods and nitrogen application on forage crop yield, quality and water use in arid area of northwest China
    WEI Zheng-Ye, ZHANG Hai-Xing, SHI Wei, CHANG Sheng-Hua, ZHANG Cheng, JIA Qian-Min, HOU Fu-Jiang
    Acta Agronomica Sinica    2022, 48 (10): 2638-2653.   DOI: 10.3724/SP.J.1006.2022.13053
    Abstract49)   HTML5)    PDF(pc) (761KB)(61)       Save

    Intercropping and nitrogen application are important measures to improve the yield and quality of forage crops. However, the suitable nitrogen application rate of silage corn/legume intercropping system in the arid area of Northwest China is not clear. Three planting methods of silage corn/Dolichos lablab intercropping (SL), silage corn/fodder soybean intercropping (SF), and silage corn monoculture (S) were conducted in this experiment. Four nitrogen application levels of 0 kg hm-2 (N1), 120 kg hm-2 (N2), 240 kg hm-2 (N3), and 360 kg hm-2 (N4) were set under each planting mode. To explore the suitable jade bean intercropping combination and nitrogen application rate in the arid area of Northwest China, the effects of planting methods and nitrogen application levels on forage crop yield, quality and water use were analyzed. The results showed that compared with S treatment, the hay yield of SL treatment increased significantly by 8.94% and 8.68% in 2019 and 2020, and the water use efficiency increased significantly by 12.28% and 8.90%, respectively. Compared with monoculture, intercropping significantly increased the contents of crude protein and crude ash, and significantly reduced the contents of starch and neutral detergent fiber. Compared with no nitrogen application, nitrogen application significantly increased hay yield, crude protein, starch, and crude fat content, significantly reduced neutral and acid detergent fiber content, but there was no significant effect on crude ash content. Compared with N1, the hay yields of N2, N3, and N4 increased significantly by 30.30%, 56.19%, and 53.95% in 2019 and 22.02%, 60.54%, and 51.83% in 2020, respectively. WUE of N3 and N4 was significantly higher than that of N1 and N2, but there was no significant difference between N3 and N4. Among all treatments, SL-N3 obtained the highest two-year average hay yield (33.10 t hm-2), crude protein content (10.01%), and WUE (62.50 kg hm-2 mm-1). To sum up, SL-N3 is a management model suitable for silage corn production in arid areas of northwest China.

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    Content diversity of phenolic compounds of waxy sorghum grains in different provinces, cities, and autonomous regions of China
    WANG Qian, LIU Shao-Xiong, CHAI Xiao-Jiao, LI Hai, ZHANG Fen, LU Ping, WANG Rui-Yun, LIU Min-Xuan
    Acta Agronomica Sinica    2022, 48 (10): 2505-2516.   DOI: 10.3724/SP.J.1006.2022.14161
    Abstract64)   HTML5)    PDF(pc) (1474KB)(57)       Save

    To comprehensively evaluate the relationship between the contents of phenolic compounds in waxy sorghum grains, the color of grains, and the source of origin in the main sorghum producing areas in China, in this study, the contents of polyphenols, flavonoids, tannin and condensed tannin in 92 waxy sorghum varieties from 15 provinces (municipalities and autonomous regions) in China were determined by Folin-Ciocalteu method, NaNO2-Al(NO3)3-NaOH method, GB/T 15686 method, and vanillin-hydrochloric acid method. The results showed that there were significant differences in the contents of polyphenols, flavonoids, tannin, and condensed tannin in different waxy sorghum varieties. The coefficient of variation ranged from 44.68% (tannin) to 79.37% (condensed tannin) and the average values were 1.25%, 1.61%, 1.41%, and 0.50%, respectively. The contents of the four phenols were consistent with the intensity of the grain color (orange-yellow > yellow > brown > red > white). There were significant differences in the contents of four phenols in waxy sorghum grains from different provinces at P < 0.05. Among them, the contents of four phenols in waxy sorghum grains in Guangxi, Guizhou, Jiangxi, and Sichuan located in southern and southwestern China were high, and the four phenols in the waxy sorghum varieties from the four areas of Liaoning, Inner Mongolia, Beijing, and Shandong located in northeastern China were low. The cumulative variance contribution of the first 3 principal components of principal component analysis reached 91.60%, among which Guangxi waxy sorghum (00013082) performed well in comprehensive traits. The contents of phenolic compounds in the core collection of waxy sorghum in China were relatively rich and there were obvious regional differences.

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    Genetic Diversity Analysis of Potato varieties
    DUAN Shao-Guang,JIN Li-Ping*,LI Guang-Cun,BIAN Chun-Song,XU Jian-Fei,HU Jun,QU Dong-Yu
    Acta Agron Sin    2017, 43 (05): 718-729.   DOI: 10.3724/SP.J.1006.2017.00718
    Abstract735)   HTML0)    PDF(pc) (8156KB)(1363)       Save

    Based on the 16 phenotype traits, we performed UPGMA cluster analysis for 454 potato accessions. All the accessions were grouped into two clusters A1 and A at the Euclidean distance of 14.66. The cluster A1 was further grouped into two subclusters A11 and A12 at the Euclidean distance of 12.74. All the accessions were grouped into nine clusters (A, B, C, H, D, E, F, G, and I) at the Euclidean distance of 11.73, and the cluster I was the biggest group, accounting for 57.5% of all the accessions. The cluster analysis more accurately revealed the morphological difference among potato materials, and distinguished the parental materials with different ecological types and genetic differences. Meanwhile, the genetic diversity of 559 potato accessions from worldwide was analyzed with 36 pairs of SSR primers. A total of 134 polymorphic alleles were amplified. Polymorphic alleles were amplified by each pair of primer ranging from one to seven, with a mean of 3.72. The fragment size amplified varied from 106 to 308 bp. The polymorphic information content values (PIC) were from 0.1545 to 0.7743 with a mean of 0.5783, which indicated SSR markers can reflect more abundant genetic diversity information in potato varieties. The further phylogenetic tree analysis showed that all 559 accessions were clustered into three groups. Group I was a mixed one, containing 133 (23.8%) materials from almost all regions. Group II was one mostly gathered by accessions from Europe, North America, Northeast and Northwest regions in China. There were 187 materials in this group, accounting for 33.5% of all. Group III consisted of 239 accessions (42.8%), mainly distributing in North America, South America, Northeast and Southwest regions in China. The clustering results based on phenotypic traits were similar to those based on SSR markers, both highly relating to geographic location, indicating that both method should be combined to use in potato genetic polymorphism evaluation.

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    Expression of CaMV 35S Promoter in Transgenic Cotton
    JIAO Gai-Li;MENG Zhao-Hong;NAN Zhi-Run;ZHANG Huan-Yang;LI Jun-Feng;WANG Jiao-Juan;ZHAO Jun-Xia;NIE An-QuanLI Yan-E;GUO San-Dui
    Acta Agron Sin    2004, 30 (11): 1135-1139.  
    Abstract2444)      PDF(pc) (368KB)(2162)       Save
    The CaMV 35S promoter is the most widely used promoter for driving transgene expression in plants. It is effective driving transgene expression in both dicots and monocots. It is usually classified as constitutive promoter. However, some reports suggest that it is not expressed in all cell and tissu
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    Cited: Baidu(12)
    Construction of SNP high-density genetic map and QTL analysis of agronomic traits in cowpea ( Vigna unguiculata (L.) Walp.)
    LI Jian-Ling, GONG Dan, WANG Su-Hua, CHEN Hong-Lin, CHENG Xu-Zhen, XIONG Tao, WANG Li-Xia
    Acta Agronomica Sinica    2022, 48 (10): 2475-2482.   DOI: 10.3724/SP.J.1006.2022.14187
    Abstract72)   HTML7)    PDF(pc) (266KB)(56)       Save

    To promote efficient utilization of cowpea germplasm resources and new gene discovery, a genetic linkage map containing 2984 Bin markers (142,146 SNPs) was constructed based on cowpea F2 population using re-sequencing technology. The map consisted of 11 linkage groups with a total length of 1333.48 cM and an average map spacing of 0.45 cM. The length of different linkage groups ranged from 84.63 cM to 183.15 cM, and the average plot distance ranged from 0.27 cM to 0.89 cM. A total of 15 QTLs were detected to be associated with 14 traits, including 100-grain weight, flower color, pod length, pod shape, pod shape and grain color, respectively. One major QTL region was detected for pod type, pod length, and number of branches per main stem, and multiple QTLs regions were detected for other traits. Candidate genes related to pod length, number of pods per plant, and single/double color of grains were further identified by gene annotation analysis in the interval. The results of QTL analysis in this study will lay a foundation for marker-assisted selection of important traits of cowpea, and candidate gene screening will help to further analyze the genetic mechanism of these traits and improve the level of molecular genetics research of cowpea.

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    Effects of wheat straw returning and tillage practices on corn yield in oasis irrigation area
    WANG Yu-Long, YU Ai-Zhong, LYU Han-Qiang, WANG Qi-Ming, SU Xiang-Xiang, CHAI Qiang
    Acta Agronomica Sinica    2022, 48 (10): 2671-2679.   DOI: 10.3724/SP.J.1006.2022.13058
    Abstract58)   HTML4)    PDF(pc) (557KB)(54)       Save

    The traditional continuous maize cropping poses challenge in increasing maize yield in the arid oasis irrigation area of northwest China. In this study, in order to determine the effects of wheat residue management and tillage practices on maize yield, a field experiment was carried out in a typical oasis irrigation region. The results indicated that different wheat straw returning methods and tillage practices had significant effects on corn dry matter accumulation and leaf area index, and there was significant in the interaction between them. During the whole growth period of maize, compared with no straw returning, straw returning treatment increased the dry matter accumulation and leaf area index of maize by 4.2%-8.2% and 7.1%-13.0%, respectively. Compared with no-tillage treatment, the conventional plow-tillage significantly increased the dry matter accumulation and leaf area index of maize by 20.2%-24.1% and 11.0%-16.0%, respectively. Straw returning methods and tillage practices had significant effects on maize grain yield and yield components (no interactions). Compared with no straw returning, the straw returning treatment significantly increased maize grain yield by 21.9%. The conventional plow-tillage significantly increased maize grain yield by 20.0% compared with no-tillage, due to an increase in spike numbers. In conclusion, conventional plow-tillage with straw returning management can be recommend as a feasible cultural method to increase the leaf area index, dry matter accumulation and yield formation of maize in the oasis irrigation areas.

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    Identification highly virulent population of soybean cyst nematode using China germplasms
    LIAN Yun, WEI He, WANG Jin-She, ZHANG Hui, LEI Chen-Fang, LI Jin-Ying, LU Wei-Guo
    Acta Agronomica Sinica    2022, 48 (10): 2443-2450.   DOI: 10.3724/SP.J.1006.2022.14188
    Abstract56)   HTML5)    PDF(pc) (1031KB)(54)       Save

    The disease caused by soybean cyst nematode (soybean cyst nematode, Heterodera glycines, SCN) is a worldwide soybean disease. The internationally used race identification models including Riggs model and Hg type for differentiate soybean cyst nematode population could not effectively distinguish the highly virulent race such as race 4, X12 population, and synthesized nematode population LY1. This study provides a simple identification method for distinguishing the three highly virulent population of the soybean cyst nematode, which would be greatly contribute to SCN research. This method included the following steps: First, we used the susceptible varieties and raised enough cysts in the soil infected highly virulent population of SCN for inoculation. Then, soybean varieties Xingxianhuipizhi (ZDD2315) and PI567516C were selected as differential hosts, and Lee was used as susceptible control for inoculation. Both ZDD2315 and PI567516C was sensitive in the inoculating reaction, indicating that the soil was infected by X12 population. If ZDD2315 was resistance while PI567516C were susceptible to the disease, indicating the soil was infected by race 4. If PI567516C was resistance to disease, it revealed that the soil was infected by LY1 population. These results indicated that the three highly virulent population including race 4, X12 population, and synthesized LY1 population could be effectively distinguished depending on the reaction of SCN on germplasms of ZDD2315 and PI567516C from China. In conclusion, these results of this study are of great significance for screening resistance germplasms, investigating the race distribution of SCN, and studying the pathogenic genes of SCN.

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    Analysis of Introgressed Segments in Near-Isogenic Lines Carrying Soybean Maturity Genes
    WEI Jin-Peng,E Wen-Di,LIU Zhang-Xiong,GUAN Rong-Xia,CHANG Ru-Zhen,QIU Li-Juan
    Acta Agron Sin    2010, 36 (2): 233-241.   DOI: 10.3724/SP.J.1006.2010.00233
    Abstract2576)      PDF(pc) (487KB)(1426)       Save
    It is very important to exploit the soybean maturity genes for developing varieties with high-yield and wide adaptability in both theory and practice. The objective of the study was to identify the size and position of introgressed segments associating with maturity genes, which provides information of fine mapping and cloning maturity genes . Twenty-three introgression lines (near isogenic lines, NILs) carrying four soybean maturity genes and their recurrent parent, were analyzed with 243 SSR markers, and 266 introgression segments were found, so each NIL containing 11.6 introgressed segments on average . The majority of 150 introgressed segments with E1, were located on chromosome 6, while 55, 49; 73 introgressed segments with E2, e3, E5 on chromosomes 20, 12 and 20 respectively. The NILs with the same maturity gene trend ed to cluster together. The results showed that E1 was related to the interval of satt643–sat_312 on chromosome 6 and sat_095 on chromosome 11; both E2 and E5 loci associated with the interval of satt587–satt496 on chromosome 20; e3 was related to the interval of satt317–satt181 on chromosome 12. Therefore, E1 locus was validated with NILs, and a new marker related to E1 was detected, and E2, e3, E5 gene-related markers were identified.
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    Cited: Baidu(3)
    Effect of Potassium Application Methods on Antioxidant Enzyme Activities, Yield and Potassium Use Efficiency of Cotton
    LI Zong-Tai, CHEN Er-Ying, ZHANG Mei-Ling, ZHAO Qiang-Long, XU Xiao-Long, JI Hong, SONG Xian-Liang, SUN Xue-Zhen
    Acta Agron Sin    2012, 38 (03): 487-494.   DOI: 10.3724/SP.J.1006.2012.00487
    Abstract2072)      PDF(pc) (394KB)(1267)       Save
    With application of modern cotton ( Gossyium hirsutum L.) varieties including Bt ( Bacillus thuringiensis) transgenic cotton, premature senescence caused by potassium (K) deficiency has become an important problem in cotton production in the Yellow River Valley of China. Field trialswere conducted in 2009 and 2010using Lumianyan 28. Four treatments consisting of no potassium (CK), basal application of potassium sulfate (T1), split application (1/2 basal dressing and 1/2 top-dressing at blossoming and boll forming stage)of potassium sulfate (T2), basal application of controlled-released compound fertilizers (T3) were designed. The same amount of potassium was applied with 150 kg K 2O ha –1 in each fertilizer treatment. The results indicated that application of potassium significantly increased chlorophyll and soluble protein contents, superoxide dismutase (SOD) and catalase (CAT) activities (at the leaf ages of 30, 40, and 60 days), seed cotton yield and lint yield, as compared with CK. Moreover, application of potassium produced significantly lower malondialdehyde (MDA) content and peoxidase (POD) activity (at the leaf ages of 30 and 45 days), as compared with CK. Split application (1/2 basal dressing and 1/2 top-dressing at blossoming and boll forming stage)of potassium sulfate significantly increased soluble protein content (at the leaf ages of 30, 40, and 60 days), SOD (at the leaf ages of 15, 30, and 45 days)and CAT(at the leaf ages of 15 and 30 days) activities, seed cotton yield, lint yield and potassium use efficiency, and produced significantly lower MDA content (at the leaf ages of 30, 45, and 60 days) and POD activity (at the leaf age of 30 days), as compared with basal application of potassium sulfate and controlled-released compound fertilizers, respectively. As far as high yield and high efficiency concerned in the experiment, the most appropriate potassium fertilization for recommendation is treatment T2 with split application (1/2 basal dressing and 1/2 top-dressing at blossoming and boll forming stage)of potassium sulfate.
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    Cited: Baidu(4)
    RNAi-mediated SMV-P3 Silencing Increases Soybean Resistance to Soybean Mosaic Virus
    YANG Xiang-Dong,NIU Lu,ZHANG Wei,YANG Jing,DU Qian,XING Guo-Jie,GUO Dong-Quan,LI Qi-Yun,DONG Ying-Shan
    Acta Agron Sin    2016, 42 (11): 1647-1655.   DOI: 10.3724/SP.J.1006.2016.01647
    Abstract373)   HTML1)    PDF(pc) (4865KB)(772)       Save

    Soybean mosaic virus (SMV) is one of the most important diseases in major soybean production areas and has severe effects on soybean production and seed quality in China. Breeding disease-resistant varieties is the most economical and effective strategy to prevent and control SMV. In this study, RNAi fragments of the gene encoding P3 protein, which is involved in SMV mobility and affecting host range, were introduced into soybean by plant-mediated RNA interference (RNAi) techniques to explore the influence of RNAi-mediated SMV-P3 silencing on soybean SMV resistance. Southern blot analysis revealed that exogenous RNAi fragments were integrated into the soybean genome at low copy numbers (1–4). T1–T3 generation transgenic soybeans were sprayed with herbicide and inserted fragments were examined using PCR. The results indicated that T-DNA insertion fragments could be stably inherited between generations of transgenic soybean. Inoculation of T2 and T3 generation transgenic soybeans with SMV suggested that transgenic soybeans exhibited significantly higher resistance to the prevailing SMV strain, SC-3, in major soybean production areas than the non-transgenic control varieties Williams 82 and SN9. The disease index was reduced by 4.37%-18.51%. Further, the resistance could be stably inherited. In conclusion, RNAi-mediated SMV-P3 silencing can significantly increase the SMV resistance of transgenic soybeans.

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    Genome-wide association study of ear cob diameter in maize
    MA Juan, CAO Yan-Yong, LI Hui-Yong
    Acta Agronomica Sinica    2021, 47 (7): 1228-1238.   DOI: 10.3724/SP.J.1006.2021.03048
    Abstract394)   HTML23)    PDF(pc) (8297KB)(441)       Save

    Maize ear cob diameter is an important trait impacting the yield of grain and cob, and the analysis of its genetic mechanism will provide a guidance for high-yield breeding. In this study, the genotypes of 309 inbred lines were identified by genotyping-by-sequencing technology. FarmCPU (fixed and random model circulating probability unification), MLMM (multiple loci mixed linear model), and CMLM (compressed mixed linear model) were used to identify significant single nucleotide polymorphisms (SNP) for ear cob diameter of Yuanyang of Henan province, Dancheng of Henan province, Yucheng of Henan province, Sanya of Hainan province in 2017 and 2019, and best linear unbiased estimate environment. A total of 12 significant SNP for ear cob diameter were detected at P < 8.60E-07. S4_29277313 was detected from Yuanyang in 2017 using FarmCPU and MLMM. The phenotypic variance explained of S1_29006330, S2_170889116, S2_2046026464, and S4_83821463 ranged from 10.23% to 14.17%, and were considered major-effect SNP. In addition, S1_29006330 was mapped in the interval of known QTL for ear cob diameter. A total of 17 candidate genes were identified. Among them, WAKL14 (wall-associated receptor kinase-like 14), transcription factor ZIM35 (zinc-finger protein expressed in inflorescence meristem 35), HMGA (HMG-Y-related protein A), histone-lysine N-methyltransferase ATX4 (Arabidopsis trithorax 4), and XTH32 (xyloglucan endotransglucosylase/hydrolase protein 32) might be important genes for ear cob diameter. The identification of four major-effect SNP and five candidate genes can provide an information for molecular marker-assisted breeding, fine mapping, and gene cloning.

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    Genetic mapping and candidate gene analysis of the major QTL cqHD2H-2 for early heading in barley ( Hordeum vulgare L.)
    YU Xin-Lian, LI Xin, YAO Xiao-Hua, YAO You-Hua, BAI Yi-Xiong, AN Li-Kun, WU Kun-Lun
    Acta Agronomica Sinica    2022, 48 (10): 2463-2474.   DOI: 10.3724/SP.J.1006.2022.11071
    Abstract72)   HTML9)    PDF(pc) (1532KB)(47)       Save

    Heading date is closely linked with agronomic performance, which is responsible for the regional and seasonal adaptation of barley varieties, and it is also a key indicator of the early or later maturity in barley. Previous studies indicated that the major QTL cqHD2H-2 for early heading was located in an 84 Mb interval on chromosome 2H of barley. To further verify the effective stability of the cqHD2H-2, in this study, we validated this locus and analyzed candidate genes. New InDel markers were developed based on the primary mapping region of cqHD2H-2 through an F5 population constructed from the cross of DZZ (early heading) and KL10 (late heading). A total of three markers tightly linked to the target gene were obtained, and one flank marker was confirmed as co-dominant marker by scanning 25 extreme early heading and 25 extreme late heading individuals of F5 population, which mapped the locus cqHD2H-2 located in a 40 Mb region between PA22 and Va07 on the chromosome 2H. The homologous gene HORVU2Hr1G087460 (HvNF-YB3) of rice heading date gene DTH8 in barley was acquired by homologous collinearity comparison of the region between barley and rice. Compared with the full length of HvNF-YB3 gene, the encoding region of Hv2H.NF-YB3 (DZZ) and Hv2H.nf-yb3 (KL10) was composed of an exon. The encoding region was 750 bp, and the promoter region was 2116 bp. There were three SNPs detected between the promoter region of Hv2H.NF-YB3 and Hv2H.nf-yb3. The relative expression levels of Hv2H.NF-YB3 and Hv2H.nf-yb3 showed that there were significant differences in the transcriptional level. Hv2H.NF-YB3 and Hv2H.nf-yb3 expressed in stem, awn, and glume, compared with Hv2H.NF-YB3, the relative expression levels of Hv2H.nf-yb3 significantly decreased in the three tissues. The relative expression levels gradually decreased with the development of the plants. In conclusion, Hv2H.NF-YB3 may play a role in the regulation of heading time in barley. The results were useful for developing early heading lines by marker-assisted selection (MAS) and laid a foundation for fine mapping and subsequent map-based cloning of cqHD2H-2.

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    Molecular Markers-assisted Selection of the Rice Blast Resistance Gene Pi-ta
    WANG Zhong-Hua;JIA Yu-Lin;WU Dian-Xing;XIA Ying-Wu
    Acta Agron Sin    2004, 30 (12): 1259-1265.  
    Abstract1705)      PDF(pc) (244KB)(1516)       Save
    The dominant molecular markers of the rice blast resistance gene Pi-ta were used to identify the presence in the Pi-ta gene of 30 breeding lines and 157 rice cultivars coming from some different countries. Meanwhile pathogenicity assays were performed with the rice blast fungus strains ZN57(IC-17) a
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    Cited: Baidu(41)
    Responses of soil diazotrophic diversity and community composition of nodulating and non-nodulating peanuts ( Arachis hypogaea L.) to nitrogen fertilization
    SUN Qi-Qi, ZHENG Yong-Mei, YU Tian-Yi, WU Yue, YANG Ji-Shun, WU Zheng-Feng, WU Ju-Xiang, LI Shang-Xia
    Acta Agronomica Sinica    2022, 48 (10): 2575-2587.   DOI: 10.3724/SP.J.1006.2022.14174
    Abstract70)   HTML7)    PDF(pc) (1143KB)(47)       Save

    Influencing mechanism of nitrogen (N) fertilization on soil diazotrophs of legumes remains unclear. Further study on the effect of different nitrogen application rates on soil nitrogen-fixing is of critical importance for high-efficiency nitrogen application of peanut field and agricultural sustainability development. We used the non-nodulating (BL) and nodulating (Huayu 22, HY22) peanuts as experimental materials, with four N application rates arranged, including N0 (without N application, 0 kg hm-2), N60 (N reduced-application, 60 kg hm-2), N120 (N common-fertilization, 120 kg hm-2), and N180 (N over-application, 180 kg hm-2). The qRT-PCR and Illumina high-throughput sequencing of nifH gene were used to analyze soil diazotrophic abundance, diversity, and community composition. Results showed that: (1) N fertilization significantly increased the contents of soil N fraction. Especially at N120, the soil microbial biomass carbon and dissolved organic carbon of nodulating peanut were significantly higher than that of non-nodulating peanut. The podding yields of non-nodulating peanut increased positively and linearly with the increasing N rates, while those of nodulating peanut was unaffected by N fertilization. (2) Under N fertilization, soil nifH copies of non-nodulating peanut were inhibited, while that of nodulating peanut decreased firstly and then increased with N rates increased. (3) N fertilization reduced firstly and then enhanced the soil diazotrophic diversity from non-nodulating peanut with N rates, while N fertilization increased firstly and then decreased that from nodulating peanut, with peak value at N120. (4) Nonrank_Bacteria and Proteobacteria were the predominated phyla. N fertilization altered the soil diazotrophic community composition of non-nodulating peanut, with dominant genera being nonrank_Bacteria (N0), unclassified_Cyanoabcteria (N60), nonrank_Bacteria (N120), and Skermanella (N180), respectively, and NO3-N being the overriding determinative factor, while exerted no effect on that of nodulating peanut (being dominated by unclassified_Proteobacteria and Skermanella except N120). The different responses of soil nitrogen-fixing bacteria to nitrogen application level between non-nodulating peanut and nodulating peanut may be due to the influence of different nitrogen sources (nitrogen fixed by nodules vs. N fertilizer). In conclusion, 120 kg hm-2 was the best for nitrogen-fixation of peanut field and thus agricultural production among the studied N rates.

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    Cloning and Expression Analysis of Fructose-1,6-Bisphosphate Aldolase Gene AhFBA1 in Peanut ( Arachis hypogaea L.)
    HEN Na,PAN Li-Juan,CHI Xiao-Yuan,CHEN Ming-Na,WANG Tong,WANG Mian,YANG Zhen,HU Dong-Qing,WANG Dao-Yuan,YU Shan-Lin
    Acta Agron Sin    2014, 40 (05): 934-941.   DOI: 10.3724/SP.J.1006.2014.00934
    Abstract676)   HTML0)    PDF(pc) (3775KB)(926)       Save

    In this article, a fructose-1,6-bisphosphate aldolase (FBA) gene was cloned from the leaf of peanut (Arachis hypogaea L. cultivar Huayu33) using RT-PCR, and was designated as AhFBA1. The whole sequence of AhFBA1 is 1489 bp and its open reading frame is 1200 bp, encoding a polypeptide of 400 amino acids. Its protein was predicted to be located in chloroplast, containing the conserved glycolytic domain. Multiple sequence alignments and phylogenetic analysis of FBA proteins indicated AhFBA1 was most similar with FBA from Glycine max, Medicago truncatula, Cicer arietinum,and Phaseolus vulgaris. The results of Real time RT-PCR showed that the expression of AhFBA1 was induced distinctly in both peanut root and leaf under salt and drought conditions, suggesting that AhFBA1 may participate in the salt and drought stress regulation of peanut. The expression of AhFBA1 was also induced by exogenous ABA in both peanut leaf and root, which indicated that AhFBA1 may regulate peanut abiotic stresses resistance through ABA-dependent pathway.

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    Cited: Baidu(1)
    Statistical analysis of randomized complete block design with repeated measure data using Generalized Linear Mixed Models (GLIMMIX)
    ZHANG Jiu-Quan, YAN Hui-Feng, CHU Ji-Deng, LI Cai-Bin
    Acta Agronomica Sinica    2021, 47 (2): 294-304.   DOI: 10.3724/SP.J.1006.2021.04085
    Abstract441)   HTML10)    PDF(pc) (340KB)(218)       Save

    Multiple measurements of the same subject are conducted, and there is autocorrelations among the data at each time point. Some special treatment is required for statistical analysis of repeated measure data. Although the repeated measure is widely used in agricultural and other research fields, the relevant and effective statistical methods are rare. In order to establish a simple, easy to use, and reliable statistical method, generalized linear mixed models (GLIMMIX) of SAS was adapted. Selection of covariance structure, variance analysis, and means comparison processes were showed by using RCB data. Traditional split plot and MANOVA methods wasted large amounts of information, reduced the power of the test, and could not handle missing data effectively, even resulting in incorrect conclusions. GLIMMIX was the best choice for variance analysis and means comparison of repeated measure data, because it was easy to use, and had powerful function, high reliability, and ability to handle missing data. At present, there was few relevant report in China, and this method would be very practical and innovative in this field.

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    Effects of increased nitrogen fertilizer on square Bt protein expression and nitrogen metabolism in cotton
    LI Han-Jia, LI Yuan, LIU Zhen-Yu, ZHANG Chen-Xia, XU Ze, WU Tian-Fan, CHEN Yuan, ZHANG Xiang, CHEN Yuan, CHEN De-Hua
    Acta Agronomica Sinica    2022, 48 (10): 2567-2574.   DOI: 10.3724/SP.J.1006.2022.14205
    Abstract53)   HTML7)    PDF(pc) (285KB)(51)       Save

    To investigate the effects of increased nitrogen application on square Bt protein concentration in cotton, the experiments were conducted at Key Laboratory of Genetics and Physiology of Yangzhou University in 2017 and 2018. The conventional cultivar Sikang 1 and hybrid cultivar Sikang 3 were used as the experimental materials. Enhanced nitrogen fertilizer rates of increased 25% to 100% nitrogen (300 (CK), 375, 450, 525, and 600 kg hm-2 as pure nitrogen) were designed to study the effect on square Bt protein content and nitrogen metabolic physiological in cotton. Compared with the control (300 kg hm-2), the squares Bt protein content increased first, but bolstered extent was reduced when increased nitrogen was at 1.50-1.75 times of conventional nitrogen rates (450-525 kg hm-2). Bt protein content in square increased by 4.5%-132.7% with the increase of 25%-100% nitrogen application. The maximum value of square Bt protein content was observed at nitrogen rate of 450-525 kg hm-2. The physiological mechanism of nitrogen metabolism showed that the trend of soluble protein (SP), free amino acid (AA), and key enzymes of protein synthesis (glutamic oxalacetic Transaminase, GOT and glutamine synthetase, GS) in squares were consistent with those of Bt protein. The activities of key enzymes of protein decomposition (protease and peptidase) decreased with the increase of nitrogen application. In conclusion, the increasing nitrogen application at optimum level in soil would promote Bt protein synthesis and benefit the insect resistance.

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    Progress on Gene Regulatory Mechanisms by Small RNAs during Plant Polyploidization
    ZHAO Xu-Bo,LI Ai-Li,MAO Long*
    Acta Agron Sin    2013, 39 (08): 1331-1338.   DOI: 10.3724/SP.J.1006.2013.01331
    Abstract897)   HTML0)    PDF(pc) (248KB)(2321)       Save

    Polyploidization is an important mechanism during plant evolution and polyploids are prevalent in the plant kingdom. Compared with their parents, polyploids often have strong growth vigor. Thus, how genes from different species are regulated in polyploid plants is a research area of great interest. Recently, small RNAs were found to be involved in re-establishing gene expression balance during plant polyploidization. In this paper, we presented an up to date overview on cis- and trans-gene regulatory mechanism in plant polyploidization that is mediated by small RNAs, especially by miRNAs and siRNAs. We also provided opinions on future directions in polyploid crop research.

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    Cited: Baidu(1)
    Inverted-Repeat Transgenic Maize Plants Resistant to Sugarcane Mosaic Virus
    BAI Yun-Feng;YANG Hong-Chun;QU Lin;ZHENG Jun;ZHANG Jin-Peng;WANG Mao-Yan;XIE Wan;ZHOU Xiao-Mei;WANG Guo-Ying
    Acta Agron Sin    2007, 33 (06): 973-978.  
    Abstract1882)      PDF(pc) (796KB)(1102)       Save

    RNA silencing is a post-transcriptional gene-silencing phenomenon induced by double-stranded RNA (dsRNA). In an attempt to generate dsRNA-mediated transgenic maize plants resistant to SCMV (sugarcane mosaic virus), we cloned SCMV NIb gene-specific sequences and inserted it into the binary vector p3301 in the sense and antisense orientations (named SCMVirNIb), which could produce RNAs capable of duplex formation in plant cells. Maize immature embryos were co-cultured with Agrobacterium carrying two vectors, one marker-free vector harboring the SCMVirNIb and one vector harboring bar gene as the selective marker. Resistant calli were recovered by selection on medium containing Biolaphos. Among the regenerated plantlets from resistant calli, 14 plants have been certified to contain SCMVirNIb by PCR amplification and DNA dot blot. T1 plants derived from the 14 plants were challenged in greenhouse with SCMV inoculums and the percentages of resistant plants in 11 T1 lines were higher than 60%. One plant in the T1 lines was found to carry SCMVirNIb without bar gene by PCR assay.
    T2 plants derived from T1 SCMV resistant transgenic plants were challenged with SCMV inoculums in field. The percentages of resistant plants from 3 lines, including the line derived from the marker-free transgenic plant, were higher than 85%. And non-transgenic control plants were all susceptive. Further molecular analysis confirmed that the resistant plants from the marker-free transgenic line contained SCMVirNIb but bar gene.

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    Genotype Analysis and QTL Mapping for Tolerance to Low Temperature in Germination by Introgression Lines in Soybean
    JIANG Hong-Wei,LI Can-Dong,LIU Chun-Yan,ZHANG Wen-Bo,QIU Peng-Cheng,et al.
    Acta Agron Sin    2009, 35 (7): 1268-1273.   DOI: 10.3724/SP.J.1006.2009.01268
    Abstract1938)      PDF(pc) (547KB)(1373)       Save
    Northeast of China is the main soybean production area. Low temperature is one of the main factors to reduce soybean yield and to limit soybean production spreading to north regions. Shortening the growth stage is considered as one way of solving the problem, but the effective way is to breed cultivars with low temperature to tolerance. The identification of low-temperature tolerance germplasm is the basis for soybean breeding. A set of backcross introgression lines were constructed with Hongfeng 11 as recurrent parent and Clark as donor parent, 46 individuals were screened out, which were more tolerant than recurrent parent Hongfeng 11 under low temperature condition. Fifty individuals from BC1F4 were used as control (random population). Fourteen QTLs on ten linkage groups for tolerance to low temperature in germination were detected with genotype analysis by chi-test and one-way ANOVA. Twelve excessive-introgression alleles from donor parent with positive effect were acquired by chi-square and 5 alleles by ANOVA. Satt237, SOYPRP1 , and Satt540 were detected by the two methods, so the three loci should be the main effect QTL and more believable. Introgression lines were used for mapping QTL related with tolerance to low temperature in germination, and the molecular mechanism could be studied in the further research on the platform established in this study for molecular assisted breeding.
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    Cited: Baidu(31)
    Dynamic Analysis on Response of Dry Matter Accumulation and Partitioning to Nitrogen Fertilizer in Wheat Cultivars with Different Plant Types
    LI Guo-Qiang,TANG Liang,ZHANG Wen-Yu,CAO Wei-Xing,ZHU Yan*
    Acta Agron Sin    2009, 35 (12): 2258-2265.   DOI: 10.3724/SP.J.1006.2009.02258
    Abstract3240)      PDF(pc) (281KB)(2373)       Save
    Biomass accumulation and partition in different plant organs affect the grain yield in cereal crops. Many investigators have established equations to simulate the dynamic growth and biomass accumulation of crops in various experiments using polynomial , Expolianear, Logistic, and Richard models. However, these models have limitations in some extent. The vapor pressure (VP) model has been used to quantitatively simulate the dynamic accumulation of dry matter in maize (Zea mays L.) roots and the branch number of princess flower . In this study, the VP model was introduced in wheat (Triticum aestivum L.) to test its applicability in simulating accumulation and partitioning of biomass. Four wheat cultivars with four plant types, respectively, were planted in 2006–2007 and 2007–2008 growing seasons under the nitrogen application levels of 75, 150, and 225 kg ha - 1 . Based on the dry weights of various organs at main growth stages, the Richards and VP equations tested the fitness of the biomass accumulation and partitioning in relation to accumulated growing-degree days. The results showed that with increasing nitrogen rate, the average dry matter growth rate (Ra), maximum growth rate (Rmax) and duration of the third phase (D3) increased, while the time reaching to Rmax was shortened consistently in four cultivars. In Aikang 58 (compact-short type), Huaimai 17 (loose type) and Yangmai 12 (intermediate type), the initial growth potential (R0) increased with the promotion of nitrogen rate, while the duration of early increment phase (D1) and the dry matter accumulation at Rmax (WRmax) decreased. In contrast, the R0, WRmax, and D1 of Ningmai 9 (compact-high type) exhibited an opposite pattern. In Aikang 58 and Ningmai 9, the second phase duration (D2) decreased when the nitrogen rate increased, and Yangmai 12 and Huaimai 17 had the smallest D2 value under the medium nitrogen rate. With the increase of nitrogen rate, the maximum dry matter partitioning percentage (Pmax) of leaf and spike in Huaimai 17 and Yangmai 12 as well as the maximum dry matter partitioning percentage of stem and sheath (PSmax) in Aikang 58 and Ningmai 9 decreased. In contrast, the Pmax of leaf and spike in Aikang 58 and Ningmai 9, and the PSmax in Huaimai 17 and Yangmai 12 increased accompanying with the increase of nitrogen rate. In terms of the maximum decreasing rate of dry matter partitioning percentage (MDRP) to leaf, Ningmai 9, Huaimai 17 and Yangmai 12 showed a decrease trend when nitrogen rate increased, while Aikang 58 was in an increase tendency. For the maximum increasing rate of dry matter partitioning percentage (MIRP) to spike, Aikang 58 and Ningmai 9 had a negative response to nitrogen rate and Huaimai 17 and Yangmai 12 had a positive response. Excessive application of nitrogen had negative effects on the promotion of MDRP to spike in Ningmai 9 and the reduction of MIRP to spike in Yangmai 12. No consistent effects were observed on the maximum changing rate of dry matter partitioning percentage to stem and sheath (RSimax and RSdmax). Thereby, plant types of wheat cultivars should be considered in the nitrogen application regime in practical system of cultivation techniques.
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    Effects of Light, Nitrogen and Their Interaction on Grain Yield and Nitrogen, Phosphorus and Potassium Absorption in Japonica Super Rice
    WANG Ya-Jiang,WEI Hai-Yan*,YAN Xi-Ting,GE Meng-Jie,MENG Tian-Yao,ZHANG Hong-Cheng,DAI Qi-Gen,HUO Zhong-Yang,XU Ke,FEI Xin-Ru
    Acta Agron Sin    2014, 40 (07): 1235-1244.   DOI: 10.3724/SP.J.1006.2014.01235
    Abstract582)   HTML0)    PDF(pc) (419KB)(969)       Save

     

    A field experiment was conducted using japonica super rice Nanjing 44 and Ningjing 3 with two nitrogen fertilization levels (N10: 150 kg ha -1, N20: 300 kg ha -1) and three shading treatments (L1: no shading, L2: shading 20 days before heading, L3: shading 20 days after heading) to study theeffects of light, nitrogen and their interaction on grain yield and NPK absorption. Results showed that yield under the same level of nitrogen fertilizer was L1>L3>L2. Compared with L1, L2 significantly reduced NPK accumulation from jointing to heading and at whole heading stage, mainly due to the significant decrease of the root oxidation ability of α-NA and root dry weight before heading, resulting in decreased root nutrient uptake capacity and the final yield decrease of 30.58%–35.26%. L3 significantly reduced NPK accumulation from heading to maturity and at whole maturity stage, mainly due to the significant decrease of the root oxidation ability of α-NA and root dry weight after heading. Although the root was gradual aging with the function declining after heading, resulting in the reduced e ffect onroot system, nutrient absorption and yield in L3 than in L2, the final yield was still 10.91% –18.47% lower in L3 than in L1. Under L2 and L3 conditions, with increasing nitrogen level, the root oxidation ability of α-NA and root dry weight increased significantly, resulting in that NPK accumulation increased significantly in various stages from jointing to maturity, then the final yield and nitrogen use efficiency improved significantly. This indicated that nitrogen fertilizer could partially offset the impact of low light stress on NPK nutrient absorption and grain yield in japonica super rice.
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    DNA Methylation Dynamic Analysis of Self Compatible Line and Self-Incompatible Line of Brassica oleracea var. acephala at Seed Germination Stage
    ZHANG Yang,HU Zhong-Ying,ZHAO Yue-Ming,LI Na,XIE Li-Nan
    Acta Agron Sin    2016, 42 (04): 532-539.   DOI: 10.3724/SP.J.1006.2016.00532
    Abstract392)   HTML0)    PDF(pc) (253KB)(513)       Save

    The seed of self-incompatible line often degraded. This studyaims to clarify the relationship between the degradation of the seeds and methylation. In this experiment, methylation sensitive amplification polymorphism (MSAP) was used to study the status and patterns of the DNA methylation at different periods in seed germination of self-incompatible line 9# and self-compatible line 14#.The improved CTAB method is adopted to extract seed germination in different periods of DNA, and then through the MSAP analysis, statistical amplification bands, compare the differences between 9# and 14#. The results are shown as follows: DNA methylation modification throughout the whole process of seed germination of 9#, at the early stage of the germination (0 to 2 days) methylation sites continued to increase; at the later stage (2 to 8 days) demethylation increased apparently, and eventually the number of demethylation was 11 times more than methylation. It was proved that DNA methylation modification was an important way to regulate the gene expression during seed germination of self-incompatible line 9#. Self-incompatible line 9# and self-compatible line 14# had different DNA methylation status clearly at the respectively periods of 0 and 2 days after seed germination.The proportion of total methylation, full-methylation and semi-methylation of 9# was all higher than of 14# at the same period. As seedings continued to grow after germination, in 9#, the proportion of full-methylation increased clearly and almost that of semi-methylation did not change, while in 14#, the proportion of semi-methylation increased clearly and almost that of full-methylation did not change.

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    QTL mapping for salt tolerance in wheat line CH7034
    ZHANG Xiao-Wen, LI Shi-Jiao, ZHANG Xiao-Jun, LI Xin, YANG Zu-Jun, ZHANG Shu-Wei, CHEN Fang, CHANG Li-Fang, GUO Hui-Juan, CHANG Zhi-Jian, QIAO Lin-Yi
    Acta Agronomica Sinica    2022, 48 (10): 2654-2662.   DOI: 10.3724/SP.J.1006.2022.11074
    Abstract58)   HTML6)    PDF(pc) (2597KB)(46)       Save

    The identification of wheat salt-tolerant germplasm is of great significance for making full use of saline land and ensuring food security. CH7034 is a wheat salt-tolerant line bred by our laboratory. In order to clarify the genetic rules and control loci for salt tolerance, QTL analysis was conducted using a recombinant inbred lines population crossed by CH7034 and salt-sensitive variety SY95-71. Based on the SNP microarray data and salt injury index, six QTL were detected on chromosomes 2A, 2D, 4B, and 5A, including QSI.sxau_2A, QSI.sxau_2D, QSI.sxau_4B.1, QSI.sxau_4B.2, QSI.sxau_5A.1, and QSI.sxau_5A.2. Among them, QSI.sxau_5A.1 was detected in all three salt stress experiments and had the highest phenotypic variance explained (15.73%-20.18%), which was different from other salt-tolerance loci reported on chromosome 5AL. Seven SSR markers were developed and integrated in the QSI.sxau_5A.1 interval, and the peak of LOD was further determined at SSR-D1. Based on the transcriptome database, 12 high-confidence genes in response to salt stress were screened from the QSI.sxau_5A.1 section. These results lay the foundation for the fine mapping and even cloning of the salt tolerance loci of CH7034, and provide new germplasm and molecular markers for the selection and breeding of wheat salt-tolerant varieties.

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    Effect of Intracellular Calcium Stores on Hypersensitive Reaction Induced by Wheat Leaf Rust Fungus
    ZHANG Bei,YAN Ai-Hua,LIU Gang,LIU Meng,HOU Chun-Yan,WANG Dong-Mei
    Acta Agron Sin    2010, 36 (05): 833-839.   DOI: 10.3724/SP.J.1006.2010.00833
    Abstract1935)      PDF(pc) (1185KB)(1191)       Save

    With injecting drugs to affect intracellular calcium stores and calcium channel in Wheat (Triticum aestivum) leaves, we investigated hypersensitive reaction (HR) changes in wheat leaves invaded by leaf rust (Puccinia triticina). The results showed that the intracellular calcium chelators (BAPTA-AM) could obviously reduce the area of dying cells caused by HR. The higher the concentration was, the smaller the area of dying cell was. While intracellular calcium activator (caffeine) was injected into wheat leaves, HR could also be detected. The HR area increased with caffeine concentration. Calcium channel blockers (heparin, RR, and 8-Br-cADPR) were separately injected into wheat leaves before inoculation. The results showed that there was a concentration-depended effect on HR with herapin, and no obviously effect with RR and 8-Br-cADPR. Therefore, we deduced that intracellular calcium might involve in the formation of calcium signal transduction through IP3 pathway during infection of wheat leaf rust fungus.

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    Cited: Baidu(4)

Co-sponsored:
the Crop Science Society of China
the Institute of Crop Science, CAAS
China Science Publishing & Media Ltd.
Published: Science Press
Editor-in-chief: Wan Jian-min
Associate Editors-in-Chief:
Chen Xiao-ya Yang Jian-chang Zhang Xian-long Wang Jian-kang Xu Ming-liang Liu Chun-ming Wang Dao-wen Sun Chuan-qing Ding Yan-feng Jin Wei-wei Chu Cheng-cai Cheng Wei-hong
Director of the editorial department:
Cheng Wei-hong
CN 11-1809/S
ISSN 0496-3490
Post subscription code: 82-336

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  • Started in 2013
  • Covered by SCIE
  • Open access in ScienceDirect

Editor in chief: Wan Jian-min
CN 10-1112/S
ISSN 2095-5421, 2214-5141(online)
Online published:
https://www.sciencedirect.com/journal/the-crop-journal
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E-mail: cropjournal@caas.cn
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