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    Heat-inducible transcription factor ZmNF-YC13 regulates heat stress response genes to improve heat tolerance in maize
    MEI Xiu-Peng, ZHAO Zi-Kun, JIA Xin-Yao, BAI Yang, LI Mei, GAN Yu-Ling, YANG Qiu-Yue, CAI Yi-Lin
    Acta Agronomica Sinica    2023, 49 (7): 1747-1757.   DOI: 10.3724/SP.J.1006.2023.23054
    Abstract397)   HTML60)    PDF(pc) (2878KB)(973)       Save

    Heat stress is an essential factor affecting maize growth and yield formation. The exploration and mechanism analysis of related tolerance genes is an important field for breeding maize heat stress tolerant varieties. However, there is few study in this aspect. In this study, we identified a nuclear factor ZmNF-YC13 associated with heat stress response, and its encoding gene expression was rapidly induced by high temperature and osmotic stress. The promoter of Arabidopsis thaliana heat stress-inducible expression gene AtHSP70 was used to drive ZmNF-YC13 and the heat-inducible expression maize material of ZmNF-YC13 (HSP21Pro:ZmNF-YC13-myc) was successfully screened. Phenotypic analysis after high temperature treatment demonstrated that leaf length, leaf width, shoot thickness, fresh and dry weight of shoot and root were significantly higher than wild type. The relative expression level showed that ZmNF-YC13 could enhance the inducible level of downstream heat stress response genes in response to heat stress. Luciferase reporter assay and ChIP-qPCR assay also revealed that ZmNF-YC13 could regulate the expression of heat stress transcription factor ZmHsfA2c. These results confirmed preliminarily that ZmNF-YC13 could improve the heat tolerance of maize by regulating downstream heat stress response genes, which could provide a theoretical basis for marker-assisted selection and germplasm identification using the polymorphism of this locus.

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    Research progress on physiological ecology and genetic basis of rice plant architecture
    XU Na, XU Quan, XU Zheng-Jin, CHEN Wen-Fu
    Acta Agronomica Sinica    2023, 49 (7): 1735-1746.   DOI: 10.3724/SP.J.1006.2023.22050
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    Rice is one of the most important crops in China and even the world. Plant type is an important agronomic trait of rice, which is closely related to yield, quality, and stress resistance. Plant type improvement has played an important role in rice breeding in the past, and will have a profound impact on rice breeding in the future. On the base of related achievements of Rice Research Institute of Shenyang Agricultural University, this paper comprehensively reviewed the research progress of rice plant type from the aspects of the concept of plant type, physiological ecology, and genetic basis of plant type breeding, and also discussed the existing problems and development direction of rice plant type. With the application of the latest research results of modern molecular biology, analyze the relationship between various organs and their optimal combination among population and individual, define the physiological ecology basis and the molecular regulatory network, and apply to plant type breeding practice, which is expected to achieve a higher level of “ideal plant type breeding” of rice.

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    Construction of evaluation method for tolerance to high-temperature and screening of heat-tolerant germplasm resources of bud stage in soybean
    LI Jia-Jia, LONG Qun, ZHU Shang-Shang, SHAN Ya-Jing, WU Mei-Yan, LU Yun, ZHI Xian-Guan, LIAO Wei, CHEN Hao-Ran, ZHAO Zhen-Bang, MIAO Long, GAO Hui-Hui, LI Ying-Hui, WANG Xiao-Bo, QIU Li-Juan
    Acta Agronomica Sinica    2023, 49 (11): 2863-2875.   DOI: 10.3724/SP.J.1006.2023.34025
    Abstract465)   HTML54)    PDF(pc) (530KB)(866)       Save

    The frequent occurrence of extreme high temperature (HT) events causes continuous heat damage to soybean production, which seriously damages the yield components and quality traits. The seeds are sensitive to the changes of the external environment at germination stage. The rising temperature and the accompanying drought will affect the emergence of soybean seeds. The establishment of a set of scientific evaluation methods for HT tolerance at bud stage can provide a theoretical basis for the early identification of soybean, the breeding of HT tolerance germplasm, and the study of tolerance mechanism. In this study, 385 germplasm resources varieties were selected as the experimental materials, which creating a HT environment by artificial climate incubator and subjected to HT-stress for 3 d (40℃, 16 h light /8 h darkness) at bud stage of soybean. Compared with the control (25℃, 16 h light /8 h darkness), the hypocotyl length of soybean bud stage was significantly decreased 10.9% under HT stress (P < 0.05). The indices of fresh root weight, dry root weight, and root-shoot ratio increased by 13.10%, 22.20%, and 16.90%, respectively (P<0.01). The results showed that HT-stress significantly affected the surface and underground biomass distribution of bud stage in soybean. Meanwhile, the principal component analysis for the coefficient of HT-tolerance for each trait converted 11 indexes into two principal component factors. The comprehensive evaluation value (H-value) of soybean response to HT-stress was obtained by the standardized analysis of membership function, and cluster analysis was conducted for the tested varieties based on H-value. Ultimately, 385 germplasm resources were divided into 5 grades for the HT-tolerance at bud stage in soybean [namely: Grade I (tolerance), Grade II (strong tolerance), Grade III (medium), Grade IV (strong sensitive), and Grade V (sensitive type)] and four HT-resistant varieties based on the specific performance (H245, H070, H268, and H216) were initially selected combined with the actual heat resistance performance. After the stepwise regression analysis of each index, a predictive model for the comprehensive evaluation of HT tolerance (H-value) at bud stage of soybean was established: H = 0.191 + 0.017X1 - 0.007X2 + 0.013X7 + 0.027X8 - 0.009X10 (R2=0.9752). Five indexes main including hypocotyl length (X1), main root length (X2), hypocotyl dry weight (X7), root fresh weight (X8), and simplified vigor index (X10) were screened out as the evaluation indexes for HT tolerance at bud stage in soybean.

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    Research progress of seed dormancy and germination regulation
    SONG Song-Quan, TANG Cui-Fang, LEI Hua-Ping, JIANG Xiao-Cheng, WANG Wei-Qing, CHENG Hong-Yan
    Acta Agronomica Sinica    2024, 50 (1): 1-15.   DOI: 10.3724/SP.J.1006.2024.34012
    Abstract765)   HTML120)    PDF(pc) (3074KB)(863)       Save

    Dormancy enables plant seeds to time germination until environmental conditions become favorable for seedling survival and growth. The dormancy characteristics of seeds are of important ecological adaptive significance and notable agricultural value. Phytohormone abscisic acid (ABA) and gibberellin (GA) are the key factors for seed dormancy and germination. Mature seeds in dormancy state contain high levels of ABA and low levels of GA. ABA induces and maintains seed dormancy, while GA antagonizes ABA and promotes seed germination. DELAY OF GERMINATION-1 (DOG1) is a major regulator of seed dormancy and had a synergistic effect with ABA to delay germination. DOG1 enhances ABA signal transduction by combining with PP2C ABA hypersensitive germination (AHG1/AHG3), and inhibits the action of AHG1 to increase ABA sensitivity and impose seed dormancy. Imprinted genes are regulated by epigenetic mechanisms before and after fertilization, and are closely related to the establishment and release of seed dormancy. In recent years, remarkable progress has been made in the regulation of seed dormancy. In the present paper, we reviewed the effects of phytohormones ABA and GA on seed dormancy and germination, the action mechanism regulating seed dormancy by DOG1, and the epigenetic regulation of seed dormancy and germination. In addition, we also propose some scientific issues that need to be further investigated in this field to provide some information for understanding the molecular mechanism of seed dormancy and germination, breeding in anti-preharvest sprouting in crop plants, and promoting the germination of dormant seeds.

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    Genetic basis analysis of high-yielding in Yannong wheat varieties
    WANG Hao, SUN Ni-Na, WANG Chu, XIAO Lu-Ning, XIAO Bei, LI Dong, LIU Jie, QIN Ran, WU Yong-Zhen, SUN Han, ZHAO Chun-Hua, LI Lin-Zhi, CUI Fa, LIU Wei
    Acta Agronomica Sinica    2023, 49 (6): 1584-1600.   DOI: 10.3724/SP.J.1006.2023.21033
    Abstract368)   HTML23)    PDF(pc) (3125KB)(813)       Save

    The Yannong series wheat has the characteristics of high yield potential, disease resistance, and wide adaptability. Yannong 1212, a high-yield and multi-resistant variety approved in recent years, has repeatedly broken the national winter wheat yield record for many times. To date, the numerous varieties derived from Yannong series have been released. Lumai 14 has derived from at least 214 wheat authorized varieties and become an important backbone parent by 2020. This objective of the study is to characterize genetic basis of high yielding potential in Yannong series, identify the key chromosomal segments of high yield and wide adaptability, and provide theoretical reference for the genetic improvement of new wheat varieties. The genotype of 38 Yannong series wheat varieties, some of their derived varieties, and 244 authorized varieties (advanced lines) were scanned by wheat 55K SNP array, and the environmental phenotypes were identified. Phenotype evaluation of yield-related traits of the natural mapping population was conducted in multiple environments. The genetic effects of the common high-frequency co-selected genetic segment (HFCS) of Yannong series varieties were characterized in detail based on the genotypes of wheat 55K SNP array. In addition, the HFCS from Lumai 14 to its derived varieties were also specified. The genetic basis of high yield potential in Yannong 1212 and Lumai 14 were revealed at the whole genome-wide level. The genetic similarity coefficients among the 17 Yannong series authorized varieties ranged from 0.80 to 0.99. Based on genotyping data from the 55K SNP array in the 17 Yannong series authorized varieties, 975 HFCS were obtained, and their segments lengths ranged from 1.00 Mb to 75.18 Mb. Most HFCS were distributed on chromosomes 2D, 4D, 6D, and 7B, with the total length accounting for more than 40% of the corresponding chromosomes, respectively. The genetic contribution rate of Lumai 14 to its 23 derived varieties was approximately 71.45% on average at the genome level, with A, B, and D of 69.63%, 66.04%, and 79.82%, respectively. A total of 430 high-frequency transmission genetic segments (HFTGS) 14 were detected in Lumai derived progency and 265 blocks (61.6%) overlapped with the HFSC in the Yannong series. Lumai 14 was a backbone parents and Yannong 1212 was a novel authorized varieties with high yield potential and wide adaptability. To characterize their genetic basis of high yield and wide adaptability, single marker analysis based on the natural population was performed. The results showed that both Lumai 14 and Yannong 1212 had been enriched in excellent alleles for thousand kernel weight (TKW) and yield per plant (YPP). Approximately 92.3% and 84.4% of the significant loci in the HFTGS of Lumai 14 were shown to increase TKW and YPP, mainly distributing on chromosomes 2A, 2B, 2D, 4A, 5B, 6A, and 7A. Yannong series authorized wheat varieties had enriched in excellent alleles of yield-related genes and QTLs especially for TKW and YPP, which played a key role to their high and stable yielding potential.

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    Genetic diversity of wheat breeding parents revealed by 55K SNP-based microarray
    LU Mao-Ang, PENG Xiao-Ai, ZHANG Ling, WANG Jian-Lai, HE Xian-Fang, ZHU Yu-Lei
    Acta Agronomica Sinica    2023, 49 (6): 1708-1714.   DOI: 10.3724/SP.J.1006.2023.21047
    Abstract339)   HTML25)    PDF(pc) (1484KB)(683)       Save

    The objective of this study is to identify the genetic diversity among wheat parental materials from different provinces. To reveal the genetic diversity and population structure by using wheat 55K SNP chip, 150 wheat accessions from Anhui, Jiangsu, Henan, Sichuan, and Shandong provinces were analyzed. A total of 52,537 SNP loci were detected in the 150 wheat accessions. 39,422 high quality markers were obtained, of which 38,135 were polymorphic, accounting for 96.74%. The distribution of 38,135 polymorphic markers among the genomes showed the least in D subgenome (10,450), the most in B subgenome (15,290). The average polymorphic information content (PIC) was 0.315, with a variation of 0.068-0.375. The averaged genetic distance of accessions differed based on the origin: Henan > Sichuan > Shandong > Jiangsu > Anhui. The results of cluster analysis, principal component analysis, and population structure analysis were highly consistent, and the clustering results were consistent with the pedigree, regional origin, and breeding group. The study revealed that the average polymorphism information content in each province was at a moderate PIC level, but the average genetic distance of the materials was close. This indicated that the high-quality germplasm resources should still be introduced to alleviate the material homogeneity, so as to increase the ability of wheat to cope with stress and reduce the vulnerability and risk in actual wheat production.

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    Genome-wide identification and characterization of MAPK genes and their response to biotic stresses in foxtail millet
    LIU Jia, ZOU Xiao-Yue, MA Ji-Fang, WANG Yong-Fang, DONG Zhi-Ping, LI Zhi-Yong, BAI Hui
    Acta Agronomica Sinica    2023, 49 (6): 1480-1495.   DOI: 10.3724/SP.J.1006.2023.24113
    Abstract379)   HTML48)    PDF(pc) (6746KB)(634)       Save

    MAPK plays an important role in plant growth and development regulation, biotic and abiotic stress responses, and hormone signal transduction. In order to elucidate the biological function of the SiMPK genes in foxtail millet, we identified the SiMPK family members in the genome and analyzed the distribution, structure, evolution, and its expression characteristics in response to different stresses. In this study, the SiMPK gene family members were identified in the genome-wide level using the amino acid sequences of conserved domains and specific TXY motifs of MAPK proteins between foxtail millet and rice. The protein physicochemical property, phylogenetic evolution, chromosome distribution, gene structure, protein conserved motif, promoter cis-acting regulatory elements, and collinearity were analyzed. The relative expression patterns of SiMPK genes in the different tissue, under the biotic stresses of Uromyces setariae-italicae Yoshino and Ostrinia furnacalis and with different hormone treatments were analyzed by qRT-PCR. The results showed that a total of 15 SiMPK genes were identified, and the encoded proteins contained 220-611 amino acids, the relative molecular weight ranged from 25.77 kD to 69.63 kD, and the isoelectric point ranged from 5.46 to 9.34. Phylogenetic analysis showed that SiMPK genes were divided into four groups. Group A, B, and C contained TEY motifs, and group D contained TDY motifs. SiMPK genes were distributed on chromosomes 1, 3, 4, 5, 8, and 9, and contained 3-11 exons. All SiMPK proteins contained motif 1 and motif 2. A number of cis-acting elements related to stress, hormones and plant growth and development were predicted in the promoter regions of the SiMPK genes. Most genes had obvious tissue expression specificity. Except for SiMPK21-2 and SiMPK6, the other members had obvious responses to 1 to 3 kinds of stresses, such as Uromyces setariae-italicae Yoshino infection, Ostrinia furnacalis feeding, and SA and MeJA treatments. The results laid a theoretical foundation for further research on the function of SiMPK genes in the biotic stresses of disease and pest in foxtail millet.

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    Types and ratios of starch granules in grains and their roles in the formation and improvement of wheat quality properties
    GAO Xin, GUO Lei, SHAN Bao-Xue, XIAO Yan-Jun, LIU Xiu-Kun, LI Hao-Sheng, LIU Jian-Jun, ZHAO Zhen-Dong, CAO Xin-You
    Acta Agronomica Sinica    2023, 49 (6): 1447-1454.   DOI: 10.3724/SP.J.1006.2023.21065
    Abstract532)   HTML74)    PDF(pc) (257KB)(621)       Save

    Wheat flour is mainly composed of protein and starch. Gluten, especially the high-molecular-weight glutenin subunit, is the key factor determining the dough quality which has been widely accepted by breeders. However, the contribution of starch composition and physicochemical properties to dough quality has not been paid enough attention. Wheat starch has a bimodal size distribution and it is composed of A-type starch granules (diameter more than 10 μm) and B-type starch granules (diameter no more than 10 μm) according to the particle size. There are differences in the physicochemical properties of different types of starch granules. Therefore, the particle size distribution of starch affects the physicochemical properties of the total wheat starch, the gluten network structure, and the gluten-starch interaction of gluten, and finally affects the dough rheological properties, and processing characteristics. In summary, from a view of starch particle distribution, this article reviewed the development and regulation mechanism, physicochemical properties of A- and B-type starch granules, and their contribution to quality and yield. Strategies for future wheat breeding were proposed, that is, the starch properties should be selected and improved. The germplasm with high B-type starch granules proportion and strong gluten-starch interaction should be screened for further utilization. The objective of this study is to provide a reference for breeding the new high-quality and strong-gluten wheat varieties.

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    A retrospective analysis of the historical evolution and developing trend of maize mega varieties in China from 1982 to 2020
    BAI Yan, GAO Ting-Ting, LU Shi, ZHENG Shu-Bo, LU Ming
    Acta Agronomica Sinica    2023, 49 (8): 2064-2076.   DOI: 10.3724/SP.J.1006.2023.23067
    Abstract474)   HTML39)    PDF(pc) (489KB)(620)       Save

    Seed is the chip of agriculture and the upgrading of excellent varieties has played an important role in improving the yield of corn in China. It is of great practical significance to study the historical contribution and developing trend of mega maize varieties in China in recent 40 years. Based on the dataset of planting area of individual maize varieties released by the National Extension and Service Center of Agricultural Technology from 1982 to 2020, 27 mega maize varieties such as Zhengdan 958 in recent 40 years with the total promotion of 321 million hectares accounting for 29.09% of the total corn planting area in China were screened..There were four utmost mega varieties (Zhengdan 958, Zhongdan 2, Danyu 13, and Xianyu 335) and six massive mega varieties (Jundan 20, Yedan 2, Nongda 108, Yedan 13, Sidan 19, and Yandan 14). The promotion period was 8-30 years, with an average of 17.63 years, the maximum promotion area in a single year was 699,700-4,569,500 hm2, with an average of 1,507,900 hm2. It was mainly distributed in Shandong, Henan, Hebei, Jilin, Heilongjiang, Inner Mongolia, and other production regions and the provinces (regions) with 13 varieties exceeding 666,700 hm2 per year were Shandong, Henan, Hebei, Jilin, Heilongjiang, Inner Mongolia, Liaoning, and Sichuan. The proportion of planting area of mega varieties to the total area increased rapidly from 1982 to 1989, remained stable from 1990 to 1997, decreased after 1998, and decreased to about 12% by 2020. Some varieties such as Yufeng 303 and Zhongkeyu 505 had the potential to develop into mega varieties. In the future, intelligent design breeding will efficiently cultivate a new generation of breakthrough varieties and accelerate the improvement of maize yield. According to the current maize production problems and future development trends, it is recommended to further improve the regional test of maize varieties in terms of distribution layout, test accuracy and variety approval standards of regional tests.

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    Identification of rice blast resistance in xian and geng germplasms by genome- wide association study
    ZHOU Hai-Ping, ZHANG Fan, CHEN Kai, SHEN Cong-Cong, ZHU Shuang-Bing, QIU Xian-Jin, XU Jian-Long
    Acta Agronomica Sinica    2023, 49 (5): 1170-1183.   DOI: 10.3724/SP.J.1006.2023.22024
    Abstract513)   HTML38)    PDF(pc) (1155KB)(605)       Save

    Rice blast is one of the major fungal diseases that threaten rice production worldwide. To improve rice blast resistance, identifying blast resistant genes and introgressing them into elite rice varieties is an effective way. In this study, a panel of 212 xian accessions and 235 geng accessions collected worldwide were evaluated for resistance against five blast isolates at seedling stage. All of them showed large variations in resistance against five isolates, and 8 xian and 12 geng accessions were detected to present resistance to all five blast isolates. Using genome-wide association strategy, a total of 43 QTLs were identified for resistance to five isolates in mix population (xian subpopulation and geng subpopulation), including 9, 4, 14, 14, and 2 QTLs for GD00-193, GD08-T19, GD17-CQ16, HB1708, and HLJ13-856, respectively. Among them, 12 resistant QTLs were detected only in xian rice sub-population, 7 only detected in geng rice sub-population, and 1 simultaneously detected in both sub-populations, indicating that blast resistance was generally better in xian than in geng rice, and there was obvious differentiation in blast resistance between xian and geng rice. A total of 11 QTLs affected resistance to two or more trains or were simultaneously identified in two or more populations, and 23 candidate genes were identified by candidate interval association analysis and haplotype analysis. Different resistance candidate genes had different frequencies in xian and geng populations. The results provide germplasm resources and favorable genes information for molecular improvement of blast resistance in rice varieties and the breeding and utilization strategies of different resistance genes.

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    Effects of nitrogen fertilizer rates on grain yield and nitrogen use efficiency of japonica-indica hybrid rice cultivar Yongyou 1540 and its physiological bases
    XU Ran, CHEN Song, XU Chun-Mei, LIU Yuan-Hui, ZHANG Xiu-Fu, WANG Dan-Ying, CHU Guang
    Acta Agronomica Sinica    2023, 49 (6): 1630-1642.   DOI: 10.3724/SP.J.1006.2023.22040
    Abstract305)   HTML36)    PDF(pc) (1154KB)(582)       Save

    The objective of this study is to understand how nitrogen (N) application rates affected on grain yield and N use efficiency (NUE) of japonica-indica hybrid rice cultivar Yongyou-1540 and its physiological bases. In the present study, a japonica-indica hybrid rice cultivar Yongyou 1540, which was widely planted in Zhejiang province was field grown in 2020 and 2021, and four N application rates [0 kg hm-2 (N0), 80 kg hm-2 (N1), 160 kg hm-2 (N2), and 240 kg hm-2 (N3)] were applied during rice growing season. The results indicated that, (1) N application rate had significant effects on grain yield and NUE of rice. Grain yield was significantly increased with the increase of N application rate from 0 kg hm-2 to 160 kg hm-2, and the increase in grain yield was mainly attributed to the increase in total spikelets per unit area. However, there were no significantly difference in grain yield between N2 and N3 treatments, which was mainly due to the reduce in grain filling percentage in N3 treatment. When compared with N2 treatment, N3 treatment significantly reduced N harvest index and NUE, including agronomic N use efficiency (AEN), N recovery efficiency (REN), N partial factor productivity (PFPN), and internal N use efficiency (IEN). (2) N application rate had significant effects on shoot growth and development. Shoot dry weight was significantly increased at jointing, heading, and maturity stages and harvest index was significantly decreased with the increase of N application rate from 0 kg hm-2 to 240 kg hm-2. The net photosynthetic rate of flag leaf, the concentration of zeatin plus zeatin riboside (Z+ZR) in flag leaves, and the activities of key enzymes involved in sucrose-to-starch conversion in grains were significantly increased with the increase of N application rate from 0 kg hm-2 to 160 kg hm-2, however, N3 treatment could not increase or even reduce the above indicators compared with N2 treatment. (3) N application rate had significant effects on rice root morphological and physiological traits. Root dry weight, root dry weight in both 0-10 cm and 10-20 cm soil layers, total root length, root length density, specific root length at jointing, heading, and maturity stages, and root oxidation activity (ROA) and concentration of Z+ZR in both roots and root-bleeding sap at mid- and late grain filling stages were significantly increased with the increase of N application rate from 0 kg hm-2 to 160 kg hm-2. However, when compared with N2 treatment, N3 treatment significantly increased root dry weight, and root dry weight in 0-10 cm soil layer, total root length and root length density. (4) The results of the correlation analysis showed that ROA, and concentration of Z+ZR in roots or in root-bleeding sap, were significantly positively correlated with net photosynthetic rate of flag leaves, the concentration of Z+ZR in flag leaves, and activities of key enzymes involved in sucrose-to-starch conversion in grains. Generally, the appropriate N application rate could improve rice root morphological and physiological traits and promote shoot growth and development, and then improved plant physiological performances during the mid- and late grain filling stages, and finally achieved the due goal of increase both grain yield and NUE.

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    ALGWAS: two-stage Adaptive Lasso-based genome-wide association study
    YANG Wen-Yu, WU Cheng-Xiu, XIAO Ying-Jie, YAN Jian-Bing
    Acta Agronomica Sinica    2023, 49 (9): 2321-2330.   DOI: 10.3724/SP.J.1006.2023.23072
    Abstract319)   HTML35)    PDF(pc) (874KB)(572)       Save

    As mainstream methods for genome-wide association analysis, mixed linear model methods have been widely used. However, the existing methods still have the problem of low detection power. In this study, a two-stage Adaptive Lasso-based genome-wide association analysis (ALGWAS) method was proposed. In the first stage, single nucleotide polymorphism (SNP) associated with target traits were screened by Adaptive Lasso, a variable selection method. In the second stage, SNPs selected from the first stage were put into the linear model as the covariates for genome-wide scanning. Compared with fastGWA, GEMMA and EMMAX, the ALGWAS method had the highest detection power and lower false discovery rate (FDR) in the simulation experiments. The above four methods were applied to genome-wide association analysis of Complete-diallel plus Unbalanced Breeding-like Inter-Cross (CUBIC) population of 1341 individuals in maize. ALGWAS method can detect the genes (ZmMADS69, ZmMADS15/31, ZmZCN8, and ZmRAP2.7) related to days to tasseling, the genes (ZmBRD1 and ZmBR2) related to plant height, and the genes (ZmUB2, ZmKRN2, and ZmCLE7) related to yield, while the other three commonly used genome-wide association analysis methods had low detection efficiency. In this study, a non-mixed linear model class of genome-wide association analysis method was proposed, which had higher detection advantage for microeffect polygenes and provided a new way for genetic analysis of complex traits.

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    Effects of light intensity and nitrogen fertilizer interaction on carbon and nitrogen metabolism at grain-filling stage and its relationship with yield and quality of southern soft japonica rice
    CHEN Xin-Yi, ZHU Ying, MA Zhong-Tao, ZHANG Ming-Yue, WEI Hai-Yan, ZHANG Hong-Cheng, LIU Guo-Dong, HU Qun, LI Guang-Yan, XU Fang-Fu
    Acta Agronomica Sinica    2023, 49 (11): 3042-3062.   DOI: 10.3724/SP.J.1006.2023.22054
    Abstract262)   HTML33)    PDF(pc) (1970KB)(570)       Save

    Southern soft japonica rice Nanjing 9108 and Yangnongxiang 28 were selected as the experimental materials, and two light intensity treatments, and four nitrogen treatments were set. Light intensity treatments [100% natural light intensity (L1) and 50% natural light intensity (L2)] and four nitrogen treatments [no nitrogen fertilizer (N1) in the middle and late growth stages, one-time nitrogen fertilizer applied at the top sixth leaf stage (N2), one-time nitrogen fertilizer applied at the top fourth leaf stage (N3), and one-time nitrogen fertilizer applied at the top second leaf stage (N4)] were conducted at grain-filling stage. The difference of carbon and nitrogen metabolism at grain-filling stage and its effects on rice yield and quality under the conditions of different light intensity and nitrogen application period as well as light-nitrogen interaction conditions were investigated. The results showed that with the decrease of light intensity at grain-filling stage the net photosynthetic rate of flag leaf decreased by 7.35%-42.36% on average, sucrose phosphate synthase (SPS), and sucrose synthase (SS) had low activity, the C/N ratio of leaves decreased by 3.98-6.49, the transportation of photosynthetic products to grains decreased, and the content of grain starch (including amylose) decreased. Meanwhile, the activities of nitrate reductase (NR), glutamine synthetase (GS), and glutamate synthetase (GOGAT) increased, plant nitrogen concentration increased, and the accumulation of protein increased relatively, which were not conducive to the formation of yield and good quality. After the application of nitrogen fertilizer at the middle and late growth stages, the activities of key enzyme in carbon and nitrogen metabolism in leaves were significantly increased, the aging of leaves was slowed down, and the grain-filling period of rice was prolonged, which were conducive to the increase of yield. With the delay of nitrogen fertilizer application period, nitrogen metabolism became more vigorous, and the protein content in grain had a relative significant increase, resulting in the decrease of the ratio of starch to protein and the ratio of amylose to protein, and the decrease of taste value. Under the experimental condition, normal light intensity combined with nitrogen fertilizer treatment (L1-N3) at the top fourth leaf stage synergistically improved the activities of key enzymes of carbon and nitrogen metabolism in leaves, thus the photosynthetic products and nitrogen-containing compounds were transported to grains in the appropriate proportions. Ultimately, the ratio of starch to protein in grain ranged from 11.43 to 12.03, and the ratio of amylose to protein ranged from 1.34 to 1.50, the rice had low hardness, high viscosity, and balance as well as good taste, high yield, and excellent quality could be obtained simultaneously.

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    Differences of physiological responses and transcriptional regulation of alfalfa with different drought tolerances under drought stresses
    CHEN Li, WANG Jing, QIU Xiao, SUN Hai-Lian, ZHANG Wen-Hao, WANG Tian-Zuo
    Acta Agronomica Sinica    2023, 49 (8): 2122-2132.   DOI: 10.3724/SP.J.1006.2023.24205
    Abstract334)   HTML36)    PDF(pc) (1732KB)(558)       Save

    Alfalfa is the most important legume forage, which is often planted in aird or semi-arid regions. The objective of this study is to explore the responsive mechanism of alfalfa with different drought tolerance to drought stress, which provides the theoretical basis for tolerant breeding of alfalfa. In this study, the new variety Medicago sativa ‘Zhongke 1’ was used as the test material, and M. sativa ‘Sanditi’ was used as the control. The effects of drought stress on growth traits, photosynthesis, chlorophyll concentration, leaf water content, osmotic adjustment substances, and antioxidant enzyme activities were determined by natural drought strategy in pots. The deferentially expressed genes were compared by transcriptome analysis. Drought stress significantly reduced plant height, biomass, relative water content of leaves, photosynthetic rate, transpiration rate, stomatal conductance, intercellular carbon dioxide and chlorophyll concentration of alfalfa. Compared with Sanditi, Zhongke 1 revealed the lower malondialdehyde concentration and electrical conductivity under drought stress, but stronger osmotic regulation ability and superoxide radical scavenging ability. Transcriptome sequencing identified 5308 and 8053 drought-responsive genes of Zhongke 1 and Sanditi, respectively. GO functional annotation demonstrated that 346 drought-responsive genes were significantly enriched in six GO item categories in Zhongke 1, while 1683 drought-responsive genes in Sanditi were significantly enriched in 29 GO item categories. The relative expression levels of 19 key drought-tolerant genes in Zhongke 1 were significantly higher than those in Sanditi. Alfalfa may maintain a relatively high photosynthetic rate by up-regulating the relative expression levels of drought tolerant genes SUS, P5CS, LEA, SOD, POD, PEPC, and NCED, thus improving osmotic regulation ability and antioxidant enzyme activity. The results provide a theoretical basis for further exploration of alfalfa’s candidate genes in response to drought stress and drought tolerant breeding.

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    Haplotype analysis of soybean leaf type regulator gene Ln and its homologous genes
    LI Gang, ZHOU Yan-Chen, XIONG Ya-Jun, CHEN Yi-Jie, GUO Qing-Yuan, GAO Jie, SONG Jian, WANG Jun, LI Ying-Hui, QIU Li-Juan
    Acta Agronomica Sinica    2023, 49 (8): 2051-2063.   DOI: 10.3724/SP.J.1006.2023.24208
    Abstract301)   HTML31)    PDF(pc) (1158KB)(540)       Save

    Soybean leaf type is an important component of ideal plant type, which is of great significance for the breeding of soybean varieties. In this study, we analyzed the haplotype of Ln and its homologous gene (Glyma.10G273800) in 598 materials from different regions of China. Ln gene and homologous gene contained three haplotypes (Hap1-Hap3), respectively. There was no significant difference in leaf length between the three haplotypes of Ln gene, and Hap2 was significantly different from Hap1/Hap3 in leaf width, leaf area, and leaf length width ratio. There were significant differences between Hap3 and Hap2 of Ln homologous gene in different leaf types. There was no difference in the ratio of leaf length to width between Hap1 and Hap2. There was no significant difference in leaf width phenotype at the 4th leaf site, and Hap1 at the 5th leaf site was significantly lower than Hap2. Leaf length, leaf area, and leaf circumference Hap1 were significantly lower than Hap2. In terms of geographical distribution, Hap2 of Ln gene was mainly distributed in high latitude and high altitude regions, and no obvious regional bias was detected in the distribution of the three haplotypes of its homologous genes. Genetic analysis showed that only D9H of Hap2 in Ln gene had artificial selection signal, while the other two mutation sites in Ln gene and two mutation sites in homologous gene had no artificial selection signal. This study provides the theoretical basis for soybean leaf type breeding and has positive significance for increasing soybean yield.

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    Yield and yield components of maize response to high plant density under reduced water and nitrogen supply
    WEI Jin-Gui, GUO Yao, CHAI Qiang, YIN Wen, FAN Zhi-Long, HU Fa-Long
    Acta Agronomica Sinica    2023, 49 (7): 1919-1929.   DOI: 10.3724/SP.J.1006.2023.23056
    Abstract278)   HTML17)    PDF(pc) (539KB)(537)       Save

    Water shortage and high fertilizer input have become the dominant factors restraining maize production in arid oasis irrigation area, it is urgent to study the technology of stable yield and increasing yield of crops with reduced water and fertilizer. To provide basis for establishing the efficient technology of stable and high yield of maize with water and nitrogen reduction, the effects of increasing density on dry matter accumulation, grain yield and yield components of maize were investigated under reduced water and nitrogen supply. A split-split plot field experiment was conducted in 2020 and 2021. Under two irrigation levels on local conventional irrigation reduced by 20% (W1) and local conventional irrigation (W2), and two levels of nitrogen fertilizer at a local conventional nitrogen reduced by 25% (N1) and local conventional nitrogen (N2), the response characteristics of dry matter accumulation and yield of maize were studied when maize density increased from 75,000 plants·hm-2 (low density, D1) by 30% (medium density D2), and by 60% (high density D3). The results showed that the grain yield of maize was significantly decreased with the reduced water and nitrogen supply, and increasing planting density by 30% could compensate the negative effect on the decrease of yield. Under the reduced water supply while maintaining N application rate, the dense planting density could significantly increase grain yield. In the two experimental years, the yield of W1 was 3.0% lower than W2. The grain yield of N1 was 12.9% lower than N2. Compared with D1, D2, and D3 increased grain yield by 12.9% and 9.2%, respectively. Compared with W2N2D1, the grain yield of W1N1D1 was decreased by 12.3%, but there was no significant difference between W2N2D1 andW1N1D2 treatments. Under the reduced water and nitrogen supply, increasing density could compensate the negative effect on the decrease of yield was mainly attributed to promoting the dry matter accumulation from early-filling to maturing stage and improving panicle number significantly. Compared with W2N2D1, the dry matter accumulation of W1N1D2 was increased by 5.8% from the early-filling to maturing stage of maize, but there were no significant differences on Vmax (maximum rate of dry matter accumulation), Vmean (mean increase rate of dry matter accumulation), Tm (the days of the maximum rate), and HI (harvest index) between W1N1D2 and W2N2D1 treatments. Compared with W2N2D1, the spike number of W1N1D2 was increased by 24.7%, but the number of kernels per spike and 1000-kernel weight of W1N1D2 were decreased by 19.3% and 14.8%, respectively. The grain yield of W1N2D2 was 13.9% higher than W2N2D1. When the nitrogen application rate was unchanged, the main reasons for the reduced irrigation, increasing density, and stable yield were the increase of dry matter accumulation, Vmean, HI, and the panicle number. Compared with W2N2D1, W1N2D2 increased panicle number, dry matter accumulation, Vmean and HI by 24.8%, 10.2%, 8.4%, and 4.7%, respectively, but there was not significant difference in 1000-kernel weight between W1N2D2 and W2N2D1 treatments. In conclusion, increasing planting density by 30% under the simultaneous reduction of water and nitrogen in the experiment was a feasible measure to save water and nitrogen for stable and high yield of maize in oasis irrigation areas. Increasing planting density by 30% was a feasible measure to save water and increase yield of maize when irrigation water was reduced by 20% while maintaining N application rate

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    Genome-wide identification and expression analysis of G2-like transcription factors family genes in wheat
    JIA Yu-Ku, GAO Hong-Huan, FENG Jian-Chao, HAO Zi-Rui, WANG Chen-Yang, XIE Ying-Xin, GUO Tian-Cai, MA Dong-Yun
    Acta Agronomica Sinica    2023, 49 (5): 1410-1425.   DOI: 10.3724/SP.J.1006.2023.21036
    Abstract407)   HTML14)    PDF(pc) (7556KB)(529)       Save

    Golden2-like (G2-like) transcription factor, a member of the GARP superfamily of MYB transcription factors, plays an important role in regulating chloroplast development. In this study, genome-wide identification of G2-like genes in wheat was carried out by bioinformatics methods, and their physicochemical properties, subcellular localization, cis-acting elements of promoters, and response patterns to abiotic stresses and hormones were analyzed. A total of 87 G2-like genes were identified from wheat, which distributed in evenly on 21 chromosomes in wheat. Phylogenetic analysis showed that these genes were divided into 14 subfamilies, and fragment replication was the main reason for the expansion of this gene family. The prediction of protein secondary structure revealed that α helix and random curl were the main amino acid sequences of G2-like gene in wheat. Promoter cis-acting elements showed that there were seven cis-acting elements (P-box, SpI, LTR, ABRE, MBS, TGA-Element, and AE-box) in 2-kb region upstream of the promoter. Among them, Ta3AG2-Like19 contained the most cis-regulatory element binding sites with a total of 18 binding sites. The qRT-PCR revealed. that the relative expression levels of Ta3AG2-like19, Ta3AG2-Like20, Ta4AG2-Like29, and Ta6AG2-Like52 were significantly up-regulated under PEG and salt stresses, and induced by GA, IAA, and ABA hormones. These genes may mediate the response of wheat plant to various abiotic stresses.

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    Effects of different soil water content on water consumption by wheat and analysis of senescence characteristics of root and flag leaf
    ZHANG Zhen, SHI Yu, ZHANG Yong-Li, YU Zhen-Wen, WANG Xi-Zhi
    Acta Agronomica Sinica    2023, 49 (7): 1895-1905.   DOI: 10.3724/SP.J.1006.2023.21052
    Abstract249)   HTML21)    PDF(pc) (633KB)(525)       Save

    The objective of this study is to clarify the effect of soil water content on wheat grain yield and the physiological reasons for its formation. During the winter wheat growing season from 2019 to 2021, four kinds of soil water content treatments were set under the field conditions of the wheat test station in Shijiawangzi village, Xiaomeng town, Yanzhou district, Shandong province: No-irrigation (W0), and the relative water content of the soil in the 0-40 cm soil layer was supplemented to 65% (W1), 75% (W2), and 85% (W3) at jointing and anthesis stages, and the winter wheat variety was Jimai 22. The effects of soil water content on water consumption, leaf and root senescence, and grain yield of wheat were studied. The results showed that the number of kernel number and 1000-kernel weight of W2 treatment were significantly higher than those of other treatments, and the highest grain yield and water use efficiency were obtained. Compared with W0, W1, and W3, the grain yield was 48.49%, 20.80%, 8.68% (2019-2020) and 46.87%, 17.36%, 7.53% (2020-2021), respectively. The water use efficiency was 21.70%, 14.25%, 15.59% (2019-2020) and 25.44%, 11.90%, 13.39% (2020-2021), respectively. Compared with the other treatments, root length density in the 40-100 cm soil layer, superoxide dismutase activity and root activity in the 40-60 cm soil layer in W2 treatment were significantly higher than those in other treatments after anthesis, and the content of malondialdehyde was significantly lower than those in other treatments. After anthesis, the superoxide dismutase activity of flag leaves in W2 treatment was significantly higher than that in W0 and W1 treatments, but there was no significant difference in W3 treatment, and the content of malondialdehyde was significantly lower than that in W0 and W1 treatments, but there was no significant difference in W3 treatment. W2 treatment increased significantly water consumption, daily water consumption, water consumption model coefficient, and soil water storage consumption in the 40-120 cm soil layer from anthesis to maturity. In conclusion, the appropriate soil moisture content could promote root growth, delay plant aging, and then improve the absorption and utilization of deep soil moisture, ensure the supply of water during grain filling, and improve significantly grain yield in wheat. Under the conditions of this experiment, the effect of W2 treatment was the best when the relative water content of 0-40 cm soil layer was increased by 75% at the jointing and anthesis stages in wheat.

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    Development, genetic deciphering, and breeding utilization of dwarf lines in foxtail millet
    DIAO Xian-Min, WANG Li-Wei, ZHI Hui, ZHANG Jun, LI Shun-Guo, CHENG Ru-Hong
    Acta Agronomica Sinica    2024, 50 (2): 265-279.   DOI: 10.3724/SP.J.1006.2024.34131
    Abstract377)   HTML42)    PDF(pc) (4944KB)(522)       Save

    Foxtail millet (Setaria italica) is an important cereal crop in northern China’s arid and semiarid dry land agriculture, which has recently been proposed as a novel model for functional genomics. Breeding dwarf varieties is the development trend of foxtail millet industry. To date, more than 70 foxtail millet dwarf lines have been developed and reported worldwide. According to morphological characteristics, foxtail millet dwarf lines can be divided into two types: compact type with erect leaves and conventional type with droopy leaves. Gibberellins (GA) sensitivity assay indicated that four materials were not sensitive to GA and the others were sensitive. Genetic analyses detected that most of the dwarf phenotype lines were controlled by recessive genes, but the height phenotype of Ai 88 was controlled by multi-dwarf-gene. So far, 79 QTL related with plant height regulation were detected by natural population GWAS or linkage analysis. Among seven genes or QTL fine mapped in foxtail millet, the semi-dominant dwarfism gene SiD1 in 84113 was the only one cloned and functionally characterized. In the history of breeding dwarf foxtail millet variety, Ai 88 was a backbone line, which had been utilized to develop 139 cultivars with reducing plant height to meet the requirement of logging resistance and mechanized harvest. In this study, we reviewed systematically the research progress of dwarfing gene in foxtail millet, sorted out the dwarfing genes that had been located and cloned, discussed the problems in the research of dwarfing genetics and breeding, and prospected the future development in foxtail.

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    Effect of water management on yield and its components of winter wheat in different precipitation years
    ZHANG Li-Hua, ZHANG Jing-Ting, DONG Zhi-Qiang, HOU Wan-Bin, ZHAI Li-Chao, YAO Yan-Rong, LYU Li-Hua, ZHAO Yi-An, JIA Xiu-Ling
    Acta Agronomica Sinica    2023, 49 (9): 2539-2551.   DOI: 10.3724/SP.J.1006.2023.21062
    Abstract252)   HTML16)    PDF(pc) (329KB)(515)       Save

    To clarify the irrigation strategy for improving yield and its components, the relationship among precipitation, irrigation, yield, and its components was systemically studied under different rainfall conditions. In this experiment, Jimai 585 used as the experimental materials, and five irrigation treatments including W0 (rain fed), W1 (jointing), W2 (jointing, flowering), W3 (jointing, flowering, filling), and W4 (overwintering, jointing, flowering, filling) in the wheat season were set up from 2010 to 2017. The results were as follows: (1) wheat grain yield varied from 6400 to 6800 kg hm-2 under different irrigation treatment in most rainfall years, and spike numbers was positively correlated with grain yield (r = 0.860*), the grain yield, grain numbers per spike, and thousand grain weight increased with the increase of irrigation amount, however, the yield increase rate decreased with each additional water (from 13.8% to 1.7%). (2) There was no obvious correlation between the total precipitation and stage precipitation and grain yield, but the impact of total precipitation on the thousand grain weight was higher than the other factors. Under the conditions of W0 and W1, the effect of total precipitation on the numbers per spike was greater than spike numbers, and vice versa after increasing irrigation. At the same time, the grain numbers per spike was significantly positively correlated with the precipitation before jointing, and the correlation between the grain numbers per spike and the precipitation from February 1 to jointing under W0 was greater than that from sowing to jointing, but it decreased with the increase of irrigation amount. With the exception of the correlation between W4 irrigation and grain numbers per spike and thousand grain weight was higher than that of precipitation, the correlation between two factors and precipitation was higher under W1-W3 conditions, which indicating that irrigation alleviate the adverse effect of insufficient precipitation on grain number per spike. (3) The number of grains per spike and thousand grain weight were the highest in a rainfall year characterized with more precipitation at early growth stages and less precipitation at later growth stages (precipitation before and after jointing 88.2 mm + 29 mm), and the highest yield was observed under 3 irrigation times, and there was no significant difference between 3 irrigation times and 2 irrigation times. In years characterized balanced rainfall (precipitation before and after jointing < 60 mm, 30-80 mm), lower and relatively stable yield and its component appeared, and the grain numbers per spike and thousand grain weight increased slightly after irrigation, but the difference in grain yield between 2 and 3 irrigation times was significant (yield increase rate: 10.5% and 22.9%) (the rainfall after jointing < 36 mm). In year characterized with less precipitation at early growth stages and more precipitation at later growth stages in (precipitation before and after jointing < 25 mm, 40 to 90 mm), the maximum grain number per spike increased by 1.5 to 7.1 grains when irrigation increased, and the grain yield of irrigation 3 times was 13.4% higher than that of irrigation 2 times when inadequate effective rainfall happened in April. In conclusion, when the precipitation before jointing was less than 60 mm (especially less than 25 mm), irrigation at jointing had an obvious effect on the increase of grain number per spike, 3 times irrigation promoted grain yield when the precipitation after jointing is less than 36 mm in a relatively balanced rainfall year type and no sufficient effective rainfall in April in years characterized with less precipitation at early stages and more precipitation at late stages, and irrigation 2 times was suggested to achieve the maximum grain yield in other rainfall years.

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    Alkaline tolerance identification method of potato seedlings and comprehensive assessment of alkaline tolerance of 86 kinds of potato germplasms
    ZHAO Peng, CHEN Guang-Xia, ZHANG Yan-Ping, YANG Xiao-Hui, LIU Fang, DONG Dao-Feng
    Acta Agronomica Sinica    2023, 49 (11): 2923-2934.   DOI: 10.3724/SP.J.1006.2023.24278
    Abstract221)   HTML28)    PDF(pc) (463KB)(498)       Save

    China is facing a serious land salinization problem. The area of saline-alkaline land reaches 100 million hectares, of which about 33 million hectares of saline-alkaline land could be used. Previous researches mostly concentrate on saline tolerance in potato, only a few research focuses on alkaline tolerance. In this study, the morphological traits such as plant height, shoot fresh weight, root fresh weight, and rooting rate of five different varieties of potato tissue culture seedlings under different concentrations of NaHCO3 simulated alkaline stress were measured, the corresponding coefficient of variation values were also calculated. Combined with coefficient of variation and the effective growth rate, the suitable simulated alkaline stress for alkaline tolerance assessment of potato tissue culture seedling was 5 mmol L-1 NaHCO3. Eighty-six kinds of tetraploid potato germplasms were treated with 5 mmol L-1 NaHCO3, alkaline tolerance coefficient of phenotypes including plant height, shoot fresh weight, root fresh weight, and rooting rate were measured. After principal component analysis and conversion by membership function, comprehensive assessment of alkaline tolerance (defined as A-value) was calculated. The A-values of Zhongshu 3 and Beifang 008 were the lowest and highest, respectively. Cluster analysis of comprehensive assessment of alkaline tolerance indicated that 86 kinds of tetraploid potato germplasms could be classified into 5 groups. Among them, the potato varieties (lines) with strong alkaline tolerance were Beifang 008, SDP632, SDP562, SDP274, SDP869, and SDP750. The regression equation between A-values and alkaline tolerance coefficients of phenotypes was established by stepwise regression analysis, A = 0.214X1 + 0.341X2 + 0.398X3 - 0.177X4 - 0.026. The four independent variables were the alkaline-tolerance coefficient of plant height, fresh weight of stem and leaf, fresh weight of root, and rooting rate. The comprehensive assessment system of alkaline tolerance of potato tissue culture seedlings established in this study could be helpful for the preliminary assessment of potato alkaline tolerance. The identified alkaline stress sensitive and tolerant gremplasms could be further used for the study of molecular regulation mechanism of alkaline stress tolerance and alkaline resistance breeding.

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    Characteristic difference in grain in-field drydown between maize cultivars with various maturation
    LI Lu-Lu, MING Bo, GAO Shang, XIE Rui-Zhi, WANG Ke-Ru, HOU Peng, XUE Jun, LI Shao-Kun
    Acta Agronomica Sinica    2023, 49 (6): 1643-1652.   DOI: 10.3724/SP.J.1006.2023.23043
    Abstract256)   HTML18)    PDF(pc) (791KB)(497)       Save

    The grain moisture concentration at harvest stage varies vastly among maize cultivars with various maturities, which is an important factor affecting mechanical grain harvesting and grain quality. Differences in maturation result in various environmental conditions for grain drying in the field, thus increasing the difficulty of comparing the characteristics of grain dehydration between cultivars. Maize cultivars with different maturities were seeded eight times at 10-day intervals from early spring to late summer in 2018 and 2019, supplying the different environmental conditions for grain in-field drying. The dynamics of grain moisture concentration were measured for all cultivars to analyze varietal differences in characterization of grain in-field drydown. Grain moisture concentration at harvest was positively correlated to growth period (r = 0.810*, 2018; r = 0.912**, 2019). Usually, the early-maturing cultivars had lower moisture concentration at harvest stage than the late-maturing cultivars. Grain moisture concentration at physiological maturity was negatively correlated to grain filling period (r = -0.484**). It was higher for early-maturing cultivars than late-maturing cultivars. Grain moisture loss rates of pre- (r = -0.655**) and post-maturity (r = -0.492**) were both inversely associated with the growth period, and were faster for early- than late-maturing cultivars. Furthermore, there was a significantly positive correlation between the grain moisture loss rate of pre-maturity and post-maturity (r = 0.466**). Overall, the cultivars with high moisture loss rate before maturity declined moisture quickly after maturity, while there was the particular cultivar with high moisture loss rate before maturity but low moisture loss rate after maturity. Duration of growth period affected grain dehydration rate. Generally, compared to late-maturing cultivar, grain of early-maturing cultivar had faster drying rates of pre- and post-maturity and lower moisture concentration at harvest stage. However, there was the noticeable case of particular cultivar when breeding and screening maize with rapid grain dehydration.

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    Yield and quality of strong gluten wheat Shiluan 02-1 under water and nitrogen interaction
    DONG Zhi-Qiang, LYU Li-Hua, YAO Yan-Rong, ZHANG Jing-Ting, ZHANG Li-Hua, YAO Hai-Po, SHEN Hai-Ping, JIA Xiu-Ling
    Acta Agronomica Sinica    2023, 49 (7): 1942-1953.   DOI: 10.3724/SP.J.1006.2023.21049
    Abstract291)   HTML14)    PDF(pc) (265KB)(495)       Save

    The purpose of this study is to explore the effects of different water and nitrogen interaction on yield and processing quality of high-quality wheat with strong gluten Shiluan 02-1, and to provide theoretical basis for how to achieve the goal of synergistically improving grain yield and processing quality through reasonable irrigation and optimal nitrogen application rate in the production of strong gluten wheat. From 2017 to 2020, the two factors split zone experiment of watering times and nitrogen application amount was set under field conditions. The main-plot factor was watering times [spring watering one time (W1, jointing water) and spring watering two times (W2, jointing water + flowering water)]; and the split-plot factor was nitrogen (N) fertilizer treatment in six levels (N0: 0, N1: 60, N2: 120, N3: 180, N4: 240, and N5: 300 kg hm-2). The study showed that: When N application rate was 0-300 kg hm-2, the yield of spring irrigating one time and spring irrigating two times increased first and then decreased with the increase of N application rate, and the N application amount corresponding to the maximum grain yield was 240 kg hm-2 in the different precipitation years. When N application rate was 120-300 kg hm-2, the yield of spring irrigating two times was significantly higher than that of spring irrigating one time. Water and N interaction had the greatest effect on the number of grains per unit area, followed by 1000-grain weight, which had the least effect on grain number per spike. When N application rate was 0-300 kg hm-2, the average value of wet gluten content, sedimentation value, water absorption rate, dough stability time, tensile energy, and maximum tensile resistance of winter wheat treated with spring irrigating two times were higher than those treated with spring irrigating one time in 2017 and 2018 (wet year). However, in 2018-2019 and 2019-2020 (drought year), it was opposite: spring irrigating one time was higher than spring irrigating two times. The wet gluten content and sedimentation value of wheat in spring irrigating one time and spring irrigating two times increased first and then decreased or gradually increased with the increase of N application rate in different precipitation years, the N application rate corresponding to the maximum of the two quality indicators was 240 kg hm-2 or 300 kg hm-2. The stabilization time, tensile energy, and maximum tensile resistance increased first and then decreased with the increase of N application rate, and reached the maximum value when N application rate was 240 kg hm-2. The grain yield and processing quality of high-quality wheat with strong gluten Shiluan 02-1 were the best when it was watered twice in spring and N was applied at 240 kg hm-2 in different precipitation years.

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    Effects of irrigation on tiller occurrence, photo-assimilates production and distribution in different stem and tillers and spike formation in wheat
    FENG Lian-Jie, YU Zhen-Wen, ZHANG Yong-Li, SHI Yu
    Acta Agronomica Sinica    2023, 49 (6): 1653-1667.   DOI: 10.3724/SP.J.1006.2022.21045
    Abstract316)   HTML25)    PDF(pc) (937KB)(493)       Save

    Moderate irrigation based on soil moisture content could increase spike number of wheat, and realize water-saving and high-yielding cultivation, but the physiological mechanism of tiller occurrence and spike formation under this condition is still unclear. A field experiment was conducted in wheat growth seasons of 2019-2020 and 2020-2021, in which the medium-spike wheat Jimai 22 and large-spike wheat Shannong 23 were used as the experimental materials. Three treatments were designed in this field experiment, including no irrigation during the whole growth period, water-saving irrigation, and sufficient irrigation. The characteristics of tiller occurrence and spike formation of wheat were studied. The results showed that the tiller node area and trans zeatin content, the photosynthetic parameters of the uppermost unfloding leaves in main stem at wintering and turn-green stage of two wheat cultivars under water-saving irrigation treatment were higher than no irrigation treatment. Under water-saving irrigation, the stem number of tillers II, III, IP, IV, and others in two spike-type wheat cultivars were increased, the total stem number per plant of Jimai 22 and Shannong 23 under W70 were 1.01 and 0.75 higher at wintering stage and 0.71 and 0.56 higher at jointing stage. The photosynthetic parameter of the uppermost unfolding leaves at jointing stage, dry matter weight and distribution amount of 13C assimilates of main stem and tillers in different positions increased significantly, the total spike number per plant of Jimai 22 and Shannong 23 were 0.36 and 0.41 higher than no irrigation in two years. The grain yield of Jimai 22 and Shannong 23 under water-saving irrigation treatment increased by 35.00% and 44.27%, and water use efficiency increased by 9.23% and 8.55% than no irrigation, respectively. Further increasing the amount of irrigation to sufficient irrigation treatment could increase the total stem number per plant at wintering and jointing stage, but there was no significant difference with water-saving irrigation in the total formation spike number per plant, spike number, and grain yield per hectare, water use efficiency was decreased. Comparison among the cultivars the total stem and spike number per plant of Jimai 22 were higher than Shannong 23, the main reason was that Jimai 22 had higher tiller node area and trans zeatin content, photosynthetic capacity in main stem, and tillers in different positions. The correlation analysis showed that the total stem number and spike number per plant, spike number, and grain yield per hectare were positively correlated with the tiller node area and trans zeatin content, and net photosynthetic rate of the uppermost unfolding leaves in main stem in two wheat cultivars, and there was significantly negatively correlated with auxin and abscisic acid content. These results indicated that the water-saving irrigation could regulate tiller occurrence and spike formation by increasing tiller node area and hormone content, improving the capacity of photo-assimilates production and distribution in main stem and tillers in different positions, thus resulting in the higher yield and water use efficiency.

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    Effects of magnesium application rate on yield and quality in oilseed rape ( Brassica napus L.)
    YE Xiao-Lei, GENG Guo-Tao, XIAO Guo-Bin, LYU Wei-Sheng, REN Tao, LU Zhi-Feng, LU Jian-Wei
    Acta Agronomica Sinica    2023, 49 (11): 3063-3073.   DOI: 10.3724/SP.J.1006.2023.34051
    Abstract244)   HTML17)    PDF(pc) (440KB)(493)       Save

    Magnesium (Mg) deficiency is one of the most serious problems in the main producing area of rapeseed in China. To evaluate the effectiveness of Mg fertilizer on seed yield and quality, field experiments were conducted at Jinxian, Jiangxi province and Anren, Hunan province during the 2020/2021 and 2021/2022 cropping seasons, with five Mg application rates (0, 15, 30, 45, and 60 kg MgO hm-2). Rapeseed yield and its components, seed Mg content, oil content, and fatty acid components were analyzed. The results showed that Mg application increased rapeseed yield by 12.0%-77.1%. Mg application rate for maximal seed yield was 21.4-45.6 kg MgO hm-2. Mg fertilizer increased the number of pods, number of seeds per pod, and 1000-weight by 5.0%-64.7%, 1.8%-19.6%, and 7.1%-8.7%, respectively, which had no significant effect on harvest density. After the application of Mg fertilizer, seed Mg concentration increased by 5.0%-30.3%, and the oil content increased by 0.63%-5.11%, but protein content reduced by 1.45%-2.34%. Seed water and glucosinolate concentration were independent of Mg nutrition. Mg application increased oil yield and protein yield per unit area by 14.4%-83.4% and 9.8%-68.1%, respectively. The amount of Mg fertilizer corresponding to the highest oil production was 30-45 MgO hm-2. As for fatty acid composition, Mg application increased the content of oleic acid and linolenic acid in seeds by 4.4%-16.0% and 3.8%-40.8%, respectively, but decreased the content of linoleic acid by 1.2%-10.1%, which had a non-significant effect on other fatty acid components. In conclusion, Mg application was crucial to the synergistic improvement of yield and quality by improving seed yield and its components (the number of pods per plant, the number of grains per pod, and 1000-grain weight), seed oil content, and the unsaturated fatty acid content. The amount of Mg fertilizer corresponding to the highest yield and oil production was 30-45 MgO hm-2.

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    Effects of combined application of nitrogen and phosphorus on yield and leaf senescence physiological characteristics in summer maize
    ZHANG Zhen-Bo, JIA Chun-Lan, REN Bai-Zhao, LIU Peng, ZHAO Bin, ZHANG Ji-Wang
    Acta Agronomica Sinica    2023, 49 (6): 1616-1629.   DOI: 10.3724/SP.J.1006.2023.23045
    Abstract320)   HTML28)    PDF(pc) (769KB)(482)       Save

    Nitrogen and phosphorus, as the essential nutrients for maize growth and development, play an important effect on maize yield. In this experiment, to investigate the effect of combined application of nitrogen and phosphorus on leaf senescence physiological and yield formation in summer maize, 11 combined applications of nitrogen and phosphorus treatments in 2020 and 15 combined applications of nitrogen and phosphorus treatments in 2021 were applied using Denhai 111 (DH111) as the test materials. The results showed that the leaf area index (LAI), the relative chlorophyll content (SPAD), and the antioxidant enzyme activities [superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)] of summer maize increased first and then decreased, and the malondialdehyde (MDA) content decreased first and then increased with the increase of nitrogen application rate under the same phosphorus application conditions. Under N0-N2 conditions, with the increasing phosphorus application, LAI, SPAD value, and antioxidant enzyme activity had an increasing trend, MDA content showed a decreasing trend and yield showed an increasing trend, while with the increasing phosphorus application, LAI, SPAD value, and antioxidant enzyme activity showed an increasing trend and then a decreasing trend, MDA content showed a decreasing trend and then an increasing trend, and yield showed an increasing trend and then a decreasing trend in N3 and N4. In 2020, the N3 P1 treatment increased yields by 2.55% compared to the N2 P3 treatment. In 2021, the N3 P1 treatment increased yields by 7.36% and 3.31% compared to the N3 P0 and N2 P2 treatments, respectively. Under the experimental conditions, the reasonable combined application of nitrogen and phosphorus (180 kg N hm-2, 60 kg P hm-2) treatment can increase the activities of antioxidant enzymes at the later stage of fertility, reduce MDA content, maintain a high leaf area index and SPAD values, increase the number of grains per ear and 1000-grain weight, ultimately leading to higher maize yield.

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    Responses of photosynthetic physiological characteristics of maize varieties released in different decades to nitrogen application rate in Jilin province
    CAO Yu-Jun, LIU Zhi-Ming, LAN Tian-Jiao, LIU Xiao-Dan, WEI Wen-Wen, YAO Fan-Yun, LYU Yan-Jie, WANG Li-Chun, WANG Yong-Jun
    Acta Agronomica Sinica    2023, 49 (8): 2183-2195.   DOI: 10.3724/SP.J.1006.2023.23058
    Abstract254)   HTML22)    PDF(pc) (619KB)(475)       Save

    It is of great practical significance to clarify the response trend of yield, leaf nitrogen content, and photosynthetic characteristics of maize varieties released in different years in Jilin province to different nitrogen levels for the breeding of high-yield maize varieties in Northeast China. In this study, six representative varieties popularized and applied in Jilin province since 1970s were used as the materials (1970s: JD101, ZD2; 1990s: SD19, JD159; 2010s: XY335, NH101). Field experiments with four nitrogen levels (0, 125, 250, and 375 kg hm-2) were set up to analyze the effects of nitrogen application on the yield, leaf nitrogen content, and photosynthetic characteristic parameters of maize varieties released in different decades. The results showed that maize grain yield under all nitrogen levels increased with the replacement of varieties, and the yield advantage of modern varieties was more obvious under high nitrogen levels. The increase of grain yield under different treatments was mainly due to the simultaneous increase of kernel numbers per ear and kernel weight. The net photosynthetic rate (Pn) of ear leaf increased with the increase of nitrogen application rate at no more than 250 kg hm-2, and the Pn of modern varieties was significantly higher than that of old varieties. When the nitrogen application rate was 375 kg hm-2, Pn decreased significantly, and the degree of reduction showed that the old varieties were higher than the modern varieties. From silking to dough stage, the decrease of Pn decreased with the increase of nitrogen application and varieties evolution. The light compensation point and dark respiration rate of maize were the highest in 2010s, the second in 1990s and the lowest in 1970s at different stages. Compared with 1990s and 1970s, the light compensation point of maize in 2010s increased by 9.72% and 27.84% on average, and the dark respiration rate increased by 7.82% and 32.98% on average. The specific leaf weight (SLW) of varieties increased with the increase of nitrogen application rate, but SLW decreased with the evolution of varieties under the same nitrogen application rate. The leaf nitrogen content per unit area (Narea) of different varieties increased with the increase of nitrogen application rate, but it was significantly decreased in the old cultivars without nitrogen application, and there was no significant difference among the cultivars treated with nitrogen application rate. The correlation analysis showed that there was a significant positive linear correlation between Pn and SLW, and the relationship between Pn and Narea can be fitted by the quadratic equation, the Narea value of Pn turning with the increase of Narea was 1.57 g m-2. The photosynthetic nitrogen use efficiency (PNUE) of all varieties decreased with the increase of nitrogen application rate, while the PNUE of cultivars at higher nitrogen levels (≥ 250 kg hm-2) increased significantly with the evolution of varieties. Thus, compared with the old varieties, the modern varieties were more beneficial to yield improvement in the higher nitrogen levels (≥ 250 kg hm-2), which was mainly attributed to the higher Pn and PNUE of the modern varieties, while the increase of Pn and PNUE was closely related to the specific leaf weight (SLM), leaf N content (Nmass, Narea), and other characters, which was recommended for reference in the breeding process of maize varieties.

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    Function and application of calcium in plant growth and development
    WANG Yu, GAO Geng-Dong, GE Meng-Meng, CHANG Ying, TAN Jing, GE Xian-Hong, WANG Jing, WANG Bo, ZHOU Guang-Sheng, FU Ting-Dong
    Acta Agronomica Sinica    2024, 50 (4): 793-807.   DOI: 10.3724/SP.J.1006.2024.34145
    Abstract429)   HTML51)    PDF(pc) (4101KB)(470)       Save

    Calcium is one of the essential elements in crops. It is widely present in roots, stems, leaves, flowers, fruits, and seeds, and is of great significance for the growth and development of crops. Calcium is a difficult element to redistribute, and its absorption and transport are subject to transpiration. Therefore, crops often experience physiological calcium deficiency, which weakens their stress resistance and reduces both yield and quality. Calcium in crops has dual functions. It not only participates in the formation of cell walls and membranes but also plays a role in responding to various environmental stimuli and internal growth and development signals as an intracellular second messenger. The absorption and transportation of calcium in cells are essential for maintaining intracellular calcium homeostasis and ensuring calcium signal transduction. In recent years, the function and application of calcium in crops have been extensively studied. In this study, we describe the distribution, absorption, transportation, and demand of calcium in crops, introduce the symptoms and causes of calcium deficiency in crops, review the nutritional structure functions of calcium, the second messenger function and the mechanism of calcium signal generation, transmission, and decoding, and summarize the role of calcium in crop growth and development, including its effects on yield, quality, and stress resistance. Meanwhile, the future research direction is proposed.

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    Advances in heat-stress responses at sexual reproduction stage in plants
    CHEN Sai-Hua, ZHONG Wei-Jie, XUE Ming
    Acta Agronomica Sinica    2023, 49 (12): 3143-3153.   DOI: 10.3724/SP.J.1006.2023.32020
    Abstract476)   HTML93)    PDF(pc) (4257KB)(468)       Save

    The occurrence of extreme hot weather poses a threat to crop production. Heat stress suffered at reproductive stage in crops is always correlated with crop yield losses, and thus the underlying molecular mechanisms are of great significance in crop thermotolerance improvement. However, relevant studies are mainly focused on Arabidopsis and less is known in crops. From the perspective of plants, here, we reviewed the heat-stress responses at reproductive stage, including meiosis process, tapetum degradation, microspore development, pollen-tube germination, and fertilization, as well as seed development. Based on these advances, we proposed feasible strategies for thermotolerance improvement, which will pave a way for the breeding of heat-tolerant crop varieties.

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    Genome-wide association analysis of cellulose content and hemicellulose content in grains of barley
    TIAN Min, LIU Xin-Chun, PAN Jia-Jia, LIANG Li-Jing, DONG Lei, LIU Mei-Chi, FENG Zong-Yun
    Acta Agronomica Sinica    2023, 49 (6): 1726-1732.   DOI: 10.3724/SP.J.1006.2023.21043
    Abstract305)   HTML26)    PDF(pc) (2148KB)(467)       Save

    Fiber is one of the important indexes to evaluate the forage quality of barley, and the analysis of its genetic mechanism has important guiding significance for the selection and breeding of forage barley. In this study, 316 barley varieties were planted in Chengdu and Kangding for two consecutive years, and 75,289 SNPs distributed in barley genome were used for genome-wide association analysis of grain cellulose and hemicellulose content, 65 and 34 significant SNPs (P ≤ 0.05/N) were repeatedly detected for cellulose and hemicellulose traits using the mixed linear model of TASSEL software, respectively. The mean explained phenotypic variation rates of markers were 13.18% and 14.10%, respectively. There were four and one significant correlation loci repeatedly detected for cellulose and hemicellulose content in three or more environments, respectively. The same SNP loci were detected for two traits, indicating that there was genetic correlation between the two traits. Six candidate genes were found for cellulose traits, and one candidate gene was found for hemicellulose traits, which laid the foundation for genetic and molecular mechanism research and genetic improvement of barley fiber.

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    Identification of OsGMS2 and construction of seed production system for genic male sterile line in rice
    TANG Jie, LONG Tuan, WU Chun-Yu, LI Xin-Peng, ZENG Xiang, WU Yong-Zhong, HUANG Pei-Jin
    Acta Agronomica Sinica    2023, 49 (8): 2025-2038.   DOI: 10.3724/SP.J.1006.2023.22051
    Abstract376)   HTML62)    PDF(pc) (15834KB)(464)       Save

    Male sterility is the basis for application of crop heterosis. The common nuclear male sterile mutant osgms2 was isolated from a mutant library created by 60Co-γ-treated indica cultivar 93-11. The male of the mutant was completely aborted, the female was nomal, and other agronomic characters were consistent with those of the wild type. Genetic analyses indicated that the male sterility phenotype was stably controlled by a single recessive gene. The OsGMS2 gene was fine-mapped within the 86 kb physical interval between two molecular markers S3b and 4826 on the long arm of chromosome 4 with a mapping population of osgms2 and Minghui 63. Further sequencing found the gene LOC_Os04g48490, encoding a fasciclin-like arabinogalactan protein, had a 9-bp deletion at position 118 to 126 from start codon of translation, resulting in three codons mutation. The osgms2 gene was a novel allele of OsFLA1. Subsequent gene knockout and genetic complementation experiments in Zhonghua 11 background confirmed the function of the gene. Real-time quantitative PCR (qPCR) analyses showed that the gene was expressed in all tissues, with the highest level at the flowering and immature seed stages. Alignment analyses revealed that the protein sequence was conserved in different species. By creating the OsGMS2-T maintainer, seed production of the common sterile line was achieved. The identification of the allelic mutant osgms2 provides the new materials for the study on rice nuclear male sterility and application of heterosis.

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    Identification and evaluation of salt-alkali tolerance and screening of salt-alkali tolerant germplasm of oat ( Avena sativa L.)
    ZHANG Jing, GAO Wen-Bo, YAN Lin, ZHANG Zong-Wen, ZHOU Hai-Tao, WU Bin
    Acta Agronomica Sinica    2023, 49 (6): 1551-1561.   DOI: 10.3724/SP.J.1006.2023.21032
    Abstract445)   HTML36)    PDF(pc) (734KB)(457)       Save

    Affected by climate change and human production activities, the world’s salt-alkali land is expanding, and soil salinization has become an important factor limiting the development of agricultural production. Oat is a crop with the strong saline-alkali tolerance. To evaluate the salinity tolerance of oat germplasm resources in China, 485 accessions were stress treated with 125 mmol L-1 NaCl, Na2SO4, and NaHCO3 (1:1:1 molar concentration) solution at germination stage. Eight growth indexes, including germination potential, germination rate, root length, bud length, root fresh weight, bud fresh weight, root dry weight, and bud dry weight, were identified at the germination stage of oats. A comprehensive evaluation and screening of oat germplasm resources for salt-alkali tolerance was performed by the correlation analysis, principal component analysis, membership function analysis, and cluster analysis. The results showed that salt-alkali inhibited all eight indexes identified, and a significant positive correlation was observed between the affiliation function values of the indexes under salt-alkali tolerance and with the comprehensive evaluation value. Eight evaluation indexes were converted into two comprehensive indexes by principal component analysis, with a cumulative variance contribution of 76.926%. The membership function analysis combined with cluster analysis screened a total of two oat accessions with high salt-alkaline tolerance (Oat 1606 and Heikowski 596) and classified 485 accessions into five classes, including two highly saline-alkaline tolerance, 49 salt-alkaline-tolerant accessions, 147 medium saline-alkaline tolerance accessions, 129 sensitive saline-alkaline accessions, and 158 high saline-alkaline sensitive accessions. The results of correlation analysis, principal component analysis, and stepwise multiple regression analysis, and the bud length was screened as the preferred indicator for the screening of salt-alkali tolerance in germinating oats, followed by root fresh weight, bud fresh weight, germination potential, and germination rate, which were also important indexes for the comprehensive evaluation and screening of salt-alkali tolerance in germinating oats.

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    Comprehensive evaluation of maize hybrids tested in Huang-Huai-Hai summer maize regional trial based on GYT biplot analysis
    YUE Hai-Wang, HAN Xuan, WEI Jian-Wei, ZHENG Shu-Hong, XIE Jun-Liang, CHEN Shu-Ping, PENG Hai-Cheng, BU Jun-Zhou
    Acta Agronomica Sinica    2023, 49 (5): 1231-1248.   DOI: 10.3724/SP.J.1006.2023.23035
    Abstract513)   HTML12)    PDF(pc) (2278KB)(457)       Save

    The objective of this study is to scientifically and accurately conduct a comprehensive evaluation of the tested hybrids participating in the Huang-Huai-Hai summer maize regional trials, and provide theoretical and practical basis for the rational distribution of hybrids and regional planning. GYT biplot analysis was applied to the data of 22 hybrids during 2020?2021 in the Huang-Huai-Hai summer maize regional trials to provide a comprehensive evaluation of the tested hybrids based on grain yield, growth period, grain moisture content at harvest, plant height, ear height, lodging rate, ear length, ear diameter, barren tip length, grain weight per ear, and hundred grain weight. The analysis of variance results showed that the genotype and environment main effects of the evaluated agronomic traits reached significant level at P < 0.05. Genotype and environment interaction effect of other traits had significant level, except for ear diameter, bald tip length, and grain weight per ear, which had no significant difference. The square sum of environmental effect on grain yield, growth period, grain moisture content, ear diameter, bare tip length, 100-seed weight, and the square sum of genotype and environment interaction effect on lodging rate were worth the largest in the square sum of total variance. The results of the correlation analysis showed that grain yield was significantly at P < 0.001 and positively correlated with 100-seed weight, plant height, ear height, ear length, ear diameter, and growth period, but negatively correlated with bald tip length. According to the GYT superiority index, Hengyu 868, Handyu 1806, and Sudan 908 had the best yield-trait combinations. The comprehensive performances of hybrids Shandan 686, Dunyu 291, Hanyu 17-6601, and Hanyu 573 were poor, and the performance of the control hybrid Zhengdan 958 was intermediate. Compared with other tested hybrids, Hengyu 868 had the widest adaptability in the Huang-Huai-Hai summer maize area, indicating outstanding regional yield advantages, and great potential for maize production in the region. Compared with the GT biplot, the GYT biplot showed that the first two principal components explained a higher proportion of variance, a better fit, and a higher reliability of the analysis results. Through GYT biplot analysis, maize hybrids with superior yield-trait combinations were identified, the GYT biplot analysis was a useful analytic tool for graphical evaluation based on multiple traits, and also set up a reference base for comprehensive evaluation of other crops.

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    Effect of nitrogen application level on grain starch accumulation at grain filling stage in sorghum spikelets
    WANG Yuan, WANG Jin-Song, DONG Er-Wei, LIU Qiu-Xia, WU Ai-Lian, JIAO Xiao-Yan
    Acta Agronomica Sinica    2023, 49 (7): 1968-1978.   DOI: 10.3724/SP.J.1006.2023.24152
    Abstract228)   HTML4)    PDF(pc) (660KB)(451)       Save

    To investigate the effect of N application level on grain-filling and starch accumulation in individual sorghum grains, sorghum variety Fenjiuliang 1 was used as the experimental material in 2019 and 2020. Six N rates of 0, 75, 150, 225, 300, and 450 kg N hm-2 were applied before sowing to experimental plots in Shanxi, China. To analyze sorghum grain-filling and starch accumulation by the Richards’ growth equation, the superior and inferior spikelets were sampled at seven days intervals at each sampling from anthesis to maturity. The rational N application level (75 kg N hm-2) showed the maximum grain number per panicle resulting in the maximum yield per hectare. For both superior and inferior spikelets, N had similar effects on grain weight, grain morphology structure, the characteristics of grain-filling, and grain starch accumulation. The grain weight, grain volume, and grain-filling rate increased with the increase of N application rate, whereas the maximum grain weight and grain volume was obtained with the zero N treatment. The grain starch accumulation rate was highly correlated with the activity of ADP-glucose pyrophosphorylase (AGPase) and soluble starch synthase (SSS). Compared to the zero N treatment, N application enhanced grain starch accumulation rate at early grain-filling stage and grain-filling rate, which may be due to the increased AGPase and SSS activity at the early grain-filling stage. Compared with rational N (75 kg N hm-2), excessive N (450 kg N hm-2) promoted grain starch accumulation by enhancing the activity of AGPase and SSS at the early grain filling stage, whereas zero N application enhanced sorghum grain weight and grain starch accumulation by extending the grain-filling duration and enhancing the activity of key enzymes in grain involved in sucrose-to-starch conversion at the late grain-filling stage.

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    Effects of water and nitrogen application on root characteristics and nitrogen utilization in winter wheat
    LIU Shi-Jie, YANG Xi-Wen, MA Geng, FENG Hao-Xiang, HAN Zhi-Dong, HAN Xiao-Jie, ZHANG Xiao-Yan, HE De-Xian, MA Dong-Yun, XIE Ying-Xin, WANG Li-Fang, WANG Chen-Yang
    Acta Agronomica Sinica    2023, 49 (8): 2296-2307.   DOI: 10.3724/SP.J.1006.2023.21051
    Abstract282)   HTML28)    PDF(pc) (709KB)(451)       Save

    The morphological and physiological characteristics of the root system determine the ability of a plant to obtain nutrients and water. In winter wheat, to building a sensible root population structure, coordinating root-shoot growth, and improving the efficiency of N fertilizer utilization, root morphological characteristic and root activity in response to water and nitrogen (N) and their relationships with dry matter accumulation in shoots, yield, and N utilization were analyzed. A field experiment was conducted using split-plot on design two factors. Two main levels of water supply, W0 (no irrigation during the whole growth period) and W1 (irrigation once each at the jointing and flowering stages), and three secondary levels of N application [N0 (0 kg hm-2), N180 (180 kg hm-2), and N300 (300 kg hm-2)] were set. Compared with W0, W1 inhibited the increase of root length density but increased the root average diameter in the 0-20 cm and 20-40 cm soil layers, increased root surface area and root dry weight density in the 0-20 cm soil layer, significantly increased root activity by 4.98%-22.7%, reduced root-shoot ratio by 1.47%-11.25%, and yield, N uptake efficiency, and partial productivity of N fertilizer increased by 15.50%, 13.40%, and 14.91%, respectively. Compared with N0, N application promoted root growth, significantly increased the average root diameter, root length density, root surface area, root dry weight density, and root activity, while decreasing the root-shoot ratio. N180 was more beneficial to root growth than N300, N180 improved root morphological indicators and activity, while increasing yield, N uptake efficiency and agronomic use efficiency of N fertilizer by 2.53%, 44.51%, and 39.37%, respectively. Correlation analysis revealed that root dry weight density from jointing stage to flowering stages was positively correlated with the yield and N use efficiency, while the root-shoot ratio was negatively correlated with yield and positively correlated with N use efficiency. Therefore, appropriate levels of irrigation and N application optimized root morphology and distribution, improved root activity, coordinated root and shoot dry matter distribution and improved yield and N utilization. In winter wheat production, W1N180 is beneficial to promote the synergistic improvement of yield and N use efficiency.

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    Effect of phosphorus fertilizer rate on rapeseed yield and quality ( Brassica napus L .)
    YAN Jin-Yao, SONG Yi, LU Zhi-Feng, REN Tao, LU Jian-Wei
    Acta Agronomica Sinica    2023, 49 (6): 1668-1677.   DOI: 10.3724/SP.J.1006.2023.24175
    Abstract360)   HTML28)    PDF(pc) (412KB)(450)       Save

    Rapeseed is an important oil crop and is sensitive to phosphorus deficiency. The soil phosphorus supply in the main rapeseed producing areas in China is poor, and phosphorus deficiency often leads to yield reduction of seed. To investigate the effects of phosphorus nutrient supply status on rapeseed yield and quality at the same time, a phosphorus fertilizer rate field experiment was conducted in two seasons of 2019-2021 in the middle reaches of Yangtze River with five treatments of 0, 45, 90, 135, and 180 kg P2O5 hm-2. The results showed that phosphorus application significantly increased the number of pods per plant, seed number, and 1000-seed weight, and thus increasing rapeseed yield. The average yield without phosphorus treatment was only 190 kg hm-2, and phosphorus application increased the yield by 8.5-12.5 times, and the maximum yields obtained according to yield effects corresponded to phosphorus applications of 51.8-65.0 kg P2O5 hm-2. The response of rapeseed phosphorus content, water content, oil content, protein, glucosinolate, oleic acid, linolenic acid, and stearic acid to phosphorus fertilizer rate reached highly significant levels, while erucic acid, linoleic acid, and palmitic acid showed less response. The oil content of rapeseed tended to increase and then decrease with increasing phosphorus application (highest at 90 kg P2O5 hm-2 and 135 kg P2O5 hm-2), protein content tended to increase slowly, and glucosinolate content decreased significantly. Excessive application of phosphorus fertilizer decreased the oleic acid content and increased the linolenic acid content of rapeseed. Path analysis showed that seed phosphorus content, oil content, and linolenic acid content had a greater direct positive effect on yield, water content, protein and linolenic acid had a greater indirect positive effect on yield through seed phosphorus content, and glucosinolate, oleic acid, linoleic acid, and stearic acid had a greater indirect negative effect on yield through seed phosphorus content. The combined results showed that the recommended phosphorus fertilizer rate for targeting rapeseed yield and edible oil quality was 45-90 kg P2O5 hm-2, and for pursuing forage cake meal protein yield, the recommended phosphorus fertilizer rate was 90-135 kg P2O5 hm-2.

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    Effects of nitrogen application on decomposition and nutrient release of returning straw, soil fertility, and maize yield
    LI Rong, MIAN You-Ming, HOU Xian-Qing, LI Pei-Fu, WANG Xi-Na
    Acta Agronomica Sinica    2023, 49 (7): 2012-2022.   DOI: 10.3724/SP.J.1006.2023.23055
    Abstract230)   HTML13)    PDF(pc) (625KB)(443)       Save

    Based on the two-year located straw returning experiment, to explore the effects of continuous nitrogen application on the decomposition and nutrient release of returning straw in the third year, soil fertility characteristics and maize yield in Yanghuang irrigation area of Ningxia, the effects of continual application rates of different pure nitrogen (0, 150, 300, and 450 kg hm-2) on decomposition, nutrient release of returning straw in the third year, soil physical and chemical properties, and maize yield were studied by nylon mesh bag method and field experiment. The results showed that nitrogen application could promote straw decomposition at 50-170 DAS (days after maize sowing), and the nitrogen application rate at 300 kg hm-2 was the best. The cumulative decomposition ratio and decomposition rate of straw under nitrogen application rate at 300 kg hm-2 compared with that no nitrogen application treatment (the control), were significantly increased by 25.6% and 20.3%, respectively. Nitrogen application could promote the nutrient release of straw, and the cumulative nutrient release rates of returning straw was ranked: potassium > carbon > nitrogen > phosphorus. The cumulative release rate of each element in all treatments increased first and then decreased with the increase of nitrogen application rate, and the effect of nitrogen application rate at 300 kg hm-2 was the best. Nitrogen application made the soil bulk density in 0-40 cm layer decrease, while the soil total porosity increased, and nitrogen application rate at 300-450 kg hm-2 was the most significant. Nitrogen application could increase the soil temperature at 20 DAS and soil water storage at 80-170 DAS, and the effect of the nitrogen application rate at 300 kg hm-2 was the best. Nitrogen application could improve soil fertility in 0-40 cm layer, and nitrogen application rate at 300 kg hm-2 had the best effect on increasing soil organic carbon, total nitrogen, alkali-hydrolyzed nitrogen, available phosphorus, and available potassium contents. Compared with the control, nitrogen application rate at 300 kg hm-2 could affect the yield composition and increase significantly maize yield by 63.7%. According to the comprehensive analysis, nitrogen application rate at 300 kg hm-2 can significantly improve soil fertility characteristics, promote the decomposition and nutrient elements release of returning straw, thus contributing to the soil fertilization and the higher yield of maize in Yanghuang irrigation area of Ningxia.

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    Identification of ZmC2s gene family and functional analysis of ZmC2-15 under heat tolerance in maize
    HUANG Yu-Jie, ZHANG Xiao-Tian, CHEN Hui-Li, WANG Hong-Wei, DING Shuang-Cheng
    Acta Agronomica Sinica    2023, 49 (9): 2331-2343.   DOI: 10.3724/SP.J.1006.2023.23069
    Abstract354)   HTML68)    PDF(pc) (22439KB)(441)       Save

    The objective of this study is to identify the members of maize ZmC2s gene family, to analyze the association between their genetic variations and heat tolerance, and to lay a foundation for clarifying its function and molecular mechanism in maize heat tolerance. Using the C2 protein domain PF00168, hmmsearch was applied to search for the members of the ZmC2s gene family from maize B73 genome. The protein isoelectric point, molecular weight, phylogenetic evolution, and gene family replication were analyzed. Using the method of candidate gene association analysis, the association between the natural variations of ZmC2s and the heat tolerance of maize seedlings was conducted, and the important heat-tolerant candidate genes of maize ZmC2s gene family were found. The relative gene expression level of the heat-tolerant candidate gene under stress was identified by Real time fluorescent quantitative PCR (RT-qPCR). The subcellular expression sites of heat-tolerant candidate gene were identified by transforming maize protoplasts. A total of 95 maize ZmC2s genes were identified from the reference genome B73 in maize. According to the order of their physical coordinates, 95 maize ZmC2s genes were named from ZmC2-1 to ZmC2-95, respectively. The length of the 95 proteins was 130-2141, the isoelectric point was 4.1-10.8, and the molecular weight was 14.1-230.1. The evolution tree of C2 gene in maize, rice, and sorghum genomes was constructed. We found that C2 genes can be divided into three major cluster branches, and each cluster branch can be subdivided into two small cluster branches. Analyzing the whole genome collinearity data of maize, rice, and sorghum, 59 ZmC2s genes were detected to have corresponding replication genes in rice and sorghum genomes. A candidate-gene based on the association analysis of ZmC2s showed that ZmC2-15/60/91 were important candidate genes for heat tolerance in maize (P ≤ 0.001, MLM), among which ZmC2-15 was the most significantly associated to heat tolerance at seedling stage (P ≤ 0.000,01, MLM), and the relative expression level of ZmC2-15 was up-regulated under various stress treatments. Subcellular localization indicated that ZmC2-15 was localized in the cytoplasm, nuclear membrane, and endoplasmic reticulum. The overexpression of ZmC2-15 improved plant heat tolerance. ZmC2-15 can be used as an important candidate gene for regulating heat tolerance in maize.

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    Formation mechanism of yield stability in high-yielding rapeseed varieties
    YU Xin-Ying, WANG Chun-Yun, LI Da-Shuang, WANG Zong-Kai, KUAI Jie, WANG Bo, WANG Jing, XU Zheng-Hua, ZHOU Guang-Sheng
    Acta Agronomica Sinica    2023, 49 (6): 1601-1615.   DOI: 10.3724/SP.J.1006.2023.24115
    Abstract301)   HTML25)    PDF(pc) (1154KB)(435)       Save

    In crop production, yield stability is as important as high yield. To explore the relationship between main agronomic traits and yield stability and to provide a theoretical basis for large-scale high yield and improved variety breeding of rapeseed, a single factor field experiments of sowing date, nitrogen rate, and planting density were carried out in Wuhan and Lanzhou. The high yielding varieties [Xiangzayou 518 (XZY 518) and Dadi 199 (DD 199)] with different yield stability were used as the materials, the agronomic, physiological indices and the yield of each treatment were measured, and the variation coefficient of each index was calculated. The results showed that: (1) Sowing date, nitrogen rate, and plant density significantly affected the rapeseed yield. There was no significant difference in the maximum yield between two varieties, however there was a significant difference in the yield stability among these treatments. The yield variation coefficients of DD 199 were lower than that of XZY 518 with the strong stability. (2) Compared with XZY518, the indices of leaf area index (LAI), light energy utilization efficiency (LUE), aboveground dry matter accumulation, and root morphology at flowering stage of DD 199 were better, while the variation coefficient of were smaller, which was the basis of high and stable yield. (3) Grey correlation analysis showed that under each treatment, the yield of rapeseed was significantly positive correlated with the population pod number, aboveground dry weight, and average root diameter at flowering stage. In addition, the variation coefficient of population pod number, aboveground dry weight, and average root diameter at flowering stage were significantly positively correlated with the variation coefficient of yield. In conclusion, optimizing the indices such as population pod number, aboveground dry weight, and average root diameter at flowering stage can provide a theoretical support for breeding of rapeseed variety and achieving high yield at large-scale.

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    Panicle silicon fertilizer optimizes the absorption and distribution of mineral elements in rice ( Oryza sativa L.) in coastal saline-alkali soil to improve salt tolerance
    WEI Hai-Min, TAO Wei-Ke, ZHOU Yan, YAN Fei-Yu, LI Wei-Wei, DING Yan-Feng, LIU Zheng-Hui, LI Gang-Hua
    Acta Agronomica Sinica    2023, 49 (5): 1339-1349.   DOI: 10.3724/SP.J.1006.2023.22031
    Abstract216)   HTML12)    PDF(pc) (2270KB)(432)       Save

    This purpose of this study is to elucidate the mechanism of silicon fertilizer on mineral element distribution at heading stage in rice. In this study, a field experiment was carried out in the coastal beach saline-alkali of Jiangsu Province (3.4 g kg-1 soil salinity, pH 8.3). The conventional japonica rice (Huaidao 5) was used as the material, and three silicon fertilizer amounts (0, 60 and 100 kg hm-2) were applied with panicle fertilizer at panicle initiation stage. The results showed that: (1) Silicon panicle fertilizer promoted plant nutrient absorption at heading stage, increased dry matter accumulation at mature stage, and increased yield, Si60 increased by 4.3% on average, Si100 increased by 8.6% on average. (2) Silicon panicle fertilizer optimized the distribution of K+ and Na+ in rice at heading stage. Silicon increase K+ content in leaves, upper sheaths and lower stems of rice, decreased Na+ content in panicles, upper leaves, sheaths and stems, and increased the K+/Na+ ratio in various tissues, thus improving ion homeostasis of rice. (3) Silicon panicle fertilizer promoted the accumulation of N, P, Ca, Mg, Fe, and Mn in leaves. Compare with Si0, the average increase of the two silicon treatments was 16.5% in P, 18.5% in Mg, 22.4% in Ca and 19.8% in Fe, and alleviated the adverse effects of saline-alkali stress on rice leaves. In summary, silicon panicle fertilizer optimizes the absorption and distribution of mineral elements in rice, reduced salt stress in young organs, promoted the accumulation of beneficial elements in leaves, improved nutrient absorption of rice, and the effect of 100 kg hm?2 was better.

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Co-sponsored:
the Crop Science Society of China
the Institute of Crop Science, CAAS
China Science Publishing & Media Ltd.
Published: Science Press
Editor-in-chief: Wan Jian-min
Associate Editors-in-Chief:
Chen Xiao-ya Yang Jian-chang Zhang Xian-long Wang Jian-kang Xu Ming-liang Liu Chun-ming Wang Dao-wen Sun Chuan-qing Ding Yan-feng Jin Wei-wei Chu Cheng-cai Cheng Wei-hong
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Yan Chun-ling
CN 11-1809/S
ISSN 0496-3490
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  • Started in 2013
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Editor in chief: Wan Jian-min
CN 10-1112/S
ISSN 2095-5421, 2214-5141(online)
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